RNA Sequence and Structure Determinants of Pol III Transcriptional Termination in Human Cells.
Verosloff MS, Corcoran WK, Dolberg TB, Bushhouse DZ, Leonard JN, Lucks JB
J Mol Biol. 2021 Jun 25;433(13):166978. doi: 10.1016/j.jmb.2021.166978. Epub 2021 Apr 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159479 | pAV-U6+27-Linear-1_PolyU(1) | Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter. |
| 159480 | pAV-U6+27-Linear-1_PolyU(2) | Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter. |
| 159481 | pAV-U6+27-Linear-1_PolyU(3) | Tests for the impact of 3 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter. |
| 159482 | pAV-U6+27-Linear-1_PolyU(4)_Tornado-Corn | Tests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter. |
| 159483 | pAV-U6+27-Linear-1_PolyU(5)_Tornado-Corn | Tests for the impact of 5 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter. |
| 159484 | pAV-U6+27-Linear-1_PolyU(6)_Tornado-Corn | Tests for the impact of 6 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter. |
| 159485 | pAV-U6+27-Linear-1_PolyU(7)_Tornado-Corn | Tests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter. |
| 159486 | pAV-U6+27-Linear-1_PolyU(8)_Tornado-Corn | Tests for the impact of 8 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter. |
| 159487 | pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn | Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter. |
| 159488 | pAV-U6+27-Linear-2_PolyU(2)_Tornado-Corn | Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter. |
| 159490 | pAV-U6+27-Linear-2_PolyU(4)_Tornado-Corn | Tests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter. |
| 159493 | pAV-U6+27-Linear-2_PolyU(7)_Tornado-Corn | Tests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter. |
| 159495 | pAV-U6+27-Linear-1_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn | Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter. |
| 159496 | pAV-U6+27-Linear-1_PolyU(4) _HairpinDistance(0)_Tornado-Corn | Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter. |
| 159498 | pAV-U6+27-Linear-1_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn | Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter. |
| 159499 | pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn | Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter. |
| 159501 | pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn | Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter. |
| 159502 | pAV-U6+27-Linear-2_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn | Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter. |
| 159503 | pAV-U6+27-Linear-3_PolyU(1)_Tornado-Corn | Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter. |
| 159504 | pAV-U6+27-Linear-3_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn | Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter. |
| 159506 | pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn | Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter. |
| 159508 | pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(0)nts_Tornado-Corn | Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter. |
| 159511 | pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(16)nts_Tornado-Corn | Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 16 nts upstream have on human polymerase III transcription elongation from a U6 promoter. |
| 159514 | pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(19)nts_Tornado-Corn | Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 19 nts upstream have on human polymerase III transcription elongation from a U6 promoter. |
| 159515 | pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(20)nts_Tornado-Corn | Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 20 nts upstream have on human polymerase III transcription elongation from a U6 promoter. |
| 166984 | GLN, Linear-3, PolyU(1) | Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter. |
| 166985 | GLN, Linear-3, PolyU(4) | Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter. |
| 166986 | GLN, Linear-3, PolyU(1), Hairpin Distance = 0nt | Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter. |
| 166987 | GLN, Linear-3, PolyU(4), Hairpin Distance = 0nt | Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter. |
| 166988 | GLY, Linear-3, PolyU(1) | Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLNYtRNA promoter. |
| 166989 | GLY, Linear-3, PolyU(4) | Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter. |
| 166990 | GLY, Linear-3, PolyU(1), Hairpin Distance = 0nt | Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter. |
| 166991 | GLY, Linear-3, PolyU(4), Hairpin Distance = 0nt | Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter. |
| 166992 | pAV-U6+27-Linear-1, PolyU(0) | Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter. |
| 166993 | pAV-U6+27Linear-1, PolyU(0), Hairpin Distance = 0nt | Tests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter. |
| 166994 | pAV-U6+27Linear-2, PolyU(0) | Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter. |
| 166995 | pAV-U6+27Linear-2, PolyU(0), Hairpin Distance = 0nt | Tests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter. |