A specific inhibitor of ALDH1A3 regulates retinoic acid biosynthesis in glioma stem cells.
Li J, Garavaglia S, Ye Z, Moretti A, Belyaeva OV, Beiser A, Ibrahim M, Wilk A, McClellan S, Klyuyeva AV, Goggans KR, Kedishvili NY, Salter EA, Wierzbicki A, Migaud ME, Mullett SJ, Yates NA, Camacho CJ, Rizzi M, Sobol RW
Commun Biol. 2021 Dec 21;4(1):1420. doi: 10.1038/s42003-021-02949-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 189741 | pLenti-PGK-EGFPc3-Neo | The construct was used to express GFP in mammalian cells as the control for the analysis of the subcellular location of this protein. |
| 189742 | pLenti-PGK-EGFP-ALDH1A1-Neo | The construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein. |
| 189743 | pLenti-PGK-EGFP-ALDH1A2-Neo | The construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein. |
| 189744 | pLenti-PGK-EGFP-ALDH1A3-Neo | The construct was used to express GFP tagged human ALDH1A3 in mammalian cells for the analysis of the subcellular location of this protein. |
| 189746 | pLenti-v2-ALDH1A3gRNA1 | The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene. |
| 189747 | pLenti-v2-ALDH1A3gRNA2 | The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene. |
| 189748 | pLenti-ALDH1A3gRNA3 | The construct was used for expression of gRNA for knocking out of the ALDH1A3 gene in Cas9 expressing cells. |
| 189749 | pDEST17-ALDH1A1 | The construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay. |
| 189750 | pDEST17-ALDH1A3 | The construct was used to express recombinant ALDH1A3 in E.Coli. The purified protein was used for activity assay. |