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Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer.

Kaku T, Sugiura K, Entani T, Osabe K, Nagai T
Sci Rep. 2021 Jul 22;11(1):14994. doi: 10.1038/s41598-021-94551-4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
197581luxA+luxB/pRSETB DuetExpressing luxA and luxB under dual T7 promoter
197582luxB:Venus+luxA/pRSETB DuetBRET lux luciferase with Venus for bacterial expression
197583luxB:cp157Venus+luxA/pRSETB DuetBRET lux luciferase with cp157Venus for bacterial expression
197584pCMVlux-cp157VenusBRET lux luciferase with cp157Venus for mammalian expression

Antibodies from Article