PGRMC1 acts as a size-selective cargo receptor to drive ER-phagic clearance of mutant prohormones.
Chen YJ, Knupp J, Arunagiri A, Haataja L, Arvan P, Tsai B
Nat Commun. 2021 Oct 13;12(1):5991. doi: 10.1038/s41467-021-26225-8.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173467 | pCMV-FLAG-PGRMC1-1-170 | PGRMC1 lacking the extreme C-terminus |
| 173468 | pCMV-FLAG-PGRMC2 | Human PGRMC2 containing an N-terminal FLAG tag |
| 173469 | pCMV-FLAG-PGRMC-Hybrid1 | N-terminal region of PGRMC2 fused to the transmembrane and C-terminal region of PGRMC1 |
| 173470 | pCMV-FLAG-PGRMC-Hybrid2 | N-terminal and transmembrane region of PGRMC2 fused to the C-terminal region of PGRMC1 |
| 173471 | pCMV-FLAG-PGRMC-Hybrid3 | N-terminal region of PGRMC1 fused to the transmembrane and C-terminal region of PGRMC2 |
| 173472 | pCMV-HA-PGRMC1-1-54 | PGRMC1 lacking the C-terminal region downstream of the transmembrane domain |
| 173473 | pCMV-HA-PGRMC1-21-195 | PGRMC1 lacking the N-terminal region upstream of the transmembrane region |