RIF1 acts in DNA repair through phosphopeptide recognition of 53BP1.
Setiaputra D, Escribano-Diaz C, Reinert JK, Sadana P, Zong D, Callen E, Sifri C, Seebacher J, Nussenzweig A, Thoma NH, Durocher D
Mol Cell. 2022 Apr 7;82(7):1359-1371.e9. doi: 10.1016/j.molcel.2022.01.025. Epub 2022 Feb 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185698 | pMX-53BP1(1-1710) 3LA | Mammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks. |
| 185707 | pMX-53BP1(1-1710) 3LA7A | Mammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity. |