Isoleucine-to-valine substitutions support cellular physiology during isoleucine deprivation.
Kok G, Schene IF, Ilcken EF, Alcaraz PS, Mendes MI, Smith DEC, Salomons G, Shehata S, Jans JJM, Maroofian R, Hoek TA, van Es RM, Rehmann H, Nieuwenhuis EES, Vos HR, Fuchs SA
Nucleic Acids Res. 2025 Jan 7;53(1):gkae1184. doi: 10.1093/nar/gkae1184.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185618 | pJET-CMV-GFP-P2A-K20-P2A-mCherry | Ribosomal stalling reporter: positive control |
| 185619 | pJET-CMV-GFP-P2A-K0-P2A-mCherry | Ribosomal stalling reporter: empty |
| 185620 | pJET-CMV-GFP-P2A-I15-P2A-mCherry | Ribosomal stalling reporter: 15x isoleucine |
| 212322 | pJet-CMV-IARS1-GFP | EGFP-tagged IARS1 |
| 212323 | pJet-CMV-IARS1[W435C]-GFP | EGFP-tagged IARS1 Trp435Cys |