Isoleucine-to-valine substitutions support cellular physiology during isoleucine deprivation
Kok G, Schene I, Ilcken E, Sobrevals P, Mendes M, Smith D, Salomons G, Shehata S, Jans J, Maroofian R, Hoek T, van Es R, Rehmann H, Nieuwenhuis E, Vos H, Fuchs S
Nucleic Acids Research
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185618 | pJET-CMV-GFP-P2A-K20-P2A-mCherry | Ribosomal stalling reporter: positive control |
| 185619 | pJET-CMV-GFP-P2A-K0-P2A-mCherry | Ribosomal stalling reporter: empty |
| 185620 | pJET-CMV-GFP-P2A-I15-P2A-mCherry | Ribosomal stalling reporter: 15x isoleucine |
| 212322 | pJet-CMV-IARS1-GFP | EGFP-tagged IARS1 |
| 212323 | pJet-CMV-IARS1[W435C]-GFP | EGFP-tagged IARS1 Trp435Cys |