Genetic Modification of Primary Human Myeloid Cells to Study Cell Migration, Activation, and Organelle Dynamics.
Greiner D, Scott TM, Olson GS, Aderem A, Roh-Johnson M, Johnson JS
Curr Protoc. 2022 Aug;2(8):e514. doi: 10.1002/cpz1.514.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185594 | pLKO-MCS | Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6. |
| 185595 | pLKO-cell-mEm | Lentiviral construct for expression of mEmerald |
| 185596 | pLKO-mito-mEm | Lentiviral construct for expression of mEmerald targeted to mitochondria |
| 185597 | pLKO-perox-mEm | Lentiviral construct for expression of mEmerald targeted to peroxisomes |
| 185598 | pLKO-cell-tagRFP | Lentiviral construct for expression of tagRFP-T |
| 185599 | pLKO-mito-tagRFP | Lentiviral construct for expression of tagRFP-T targeted to mitochondria |
| 185600 | pLKO-perox-tagRFP | Lentiviral construct for expression of tagRFP-T targeted to peroxisomes |
| 185601 | pLKO-cell-mCer | Lentiviral construct for expression of mCerulean |
| 185602 | pLKO-mito-mCer | Lentiviral construct for expression of mCerulean targeted to mitochondria |
| 185603 | pLKO-perox-mCer | Lentiviral construct for expression of mCerulean targeted to peroxisomes |