Engineering cyanobacterial chassis for improved electron supply toward a heterologous ene-reductase.
Spasic J, Oliveira P, Pacheco C, Kourist R, Tamagnini P
J Biotechnol. 2022 Dec 10;360:152-159. doi: 10.1016/j.jbiotec.2022.11.005. Epub 2022 Nov 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185532 | pGEM_ΔndhD2.km | a pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locus |
| 185533 | pGEM_Δflv3.sm | a pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locus |