Expression and activity of heterologous hydroxyisocaproate dehydrogenases in Synechocystis sp. PCC 6803 DeltahoxYH.
Jurkas V, Winkler CK, Poschenrieder S, Oliveira P, Pacheco CC, Ferreira EA, Weissensteiner F, De Santis P, Kara S, Kourist R, Tamagnini P, Kroutil W
Eng Microbiol. 2021 Nov 26;2(1):100008. doi: 10.1016/j.engmic.2021.100008. eCollection 2022 Mar.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185453 | pET21a(+)_co_L-HicDH | CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+). |
| 185456 | pSEVA251_ Ptrc.x.tetO2::D-HicDH | CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid. |