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Expression and activity of heterologous hydroxyisocaproate dehydrogenases in Synechocystis sp. PCC 6803 ΔhoxYH

Jurkaš V, Winkler CK, Poschenrieder S, Oliveira P, Pacheco CC, Ferreira EA, Weissensteiner F, De Santis P, Kara S, Kourist R, Tamagnini P, Kroutil W
Engineering Microbiology, 2022 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
185453pET21a(+)_co_L-HicDHCDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).
185456pSEVA251_ Ptrc.x.tetO2::D-HicDHCDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.

Antibodies from Article