Expression and activity of heterologous hydroxyisocaproate dehydrogenases in Synechocystis sp. PCC 6803 ΔhoxYH
Jurkaš V, Winkler CK, Poschenrieder S, Oliveira P, Pacheco CC, Ferreira EA, Weissensteiner F, De Santis P, Kara S, Kourist R, Tamagnini P, Kroutil W
Engineering Microbiology, 2022
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
185453 | pET21a(+)_co_L-HicDH | CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+). |
185456 | pSEVA251_ Ptrc.x.tetO2::D-HicDH | CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid. |