Compartmentalization of telomeres through DNA-scaffolded phase separation.
Jack A, Kim Y, Strom AR, Lee DSW, Williams B, Schaub JM, Kellogg EH, Finkelstein IJ, Ferro LS, Yildiz A, Brangwynne CP
Dev Cell. 2022 Jan 24;57(2):277-290.e9. doi: 10.1016/j.devcel.2021.12.017.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185443 | pOmniBac zz TEV YBBR TRF2 | Expresses human TRF2 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells |
| 185444 | pOmniBac zz TEV YBBR POT1 | Expresses human POT1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells |
| 185445 | pOmniBac zz TEV YBBR TRF1 | Expresses human TRF1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells |
| 185446 | pBig1a zz TEV YBBR TPP1 MBP TEV TIN2 | Coexpresses human TPP1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site and human TIN2 with an MBP affinity tag and TEV cleavage site in insect cells |
| 185447 | pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1) | Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cells |
| 185448 | pBig1a zz TEV YBBR POT1 MBP TEV TPP1 MBP TEV TIN2 ZZ TEV TRF2 (4comp2) | Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF2 with a zz tag and TEV site, and human TIN2 and TPP1 each with an MBP affinity tag and TEV site in insect cells |
| 185449 | pBig1a zz TEV YBBR RAP1 ZZ TEV TRF2 | Coexpresses human RAP1 with an MBP affinity tag, YBBR site, and TEV site, and human TRF2 with a zz tag and TEV site |