Eliminating genes for a two-component system increases PHB productivity in Cupriavidus basilensis 4G11 under PHB suppressing, nonstress conditions.
Sander K, Abel AJ, Friedline S, Sharpless W, Skerker J, Deutschbauer A, Clark DS, Arkin AP
Biotechnol Bioeng. 2024 Jan;121(1):139-156. doi: 10.1002/bit.28532. Epub 2023 Aug 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185390 | RS35075_pMQ30_KO_Kan | Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11 |
| 185391 | RS02060_pMQ30_KO_Kan | Non-replicating vector used to create markerless deletion of RR42_RS02060 in C. basilensis 4G11 |
| 185392 | RS28935_pMQ30_KO_Kan | Non-replicating vector used to create markerless deletion of RR42_RS28935 in C. basilensis 4G11 |
| 185393 | RS18960_pMQ30_KO_Kan | Non-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11 |
| 185394 | RS18965_pMQ30_KO_Kan | Non-replicating vector used to create markerless deletion of RR42_RS18965 in C. basilensis 4G11 |
| 185395 | RS18970_pMQ30_KO_Kan | Non-replicating vector used to create markerless deletion of RR42_RS18970 in C. basilensis 4G11 |
| 185396 | RS17060_pMQ30_KO_Kan | Non-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11 |
| 185398 | RS17055_pMQ30_KO_Kan | Non-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11 |