Natural and Designed Proteins Inspired by Extremotolerant Organisms Can Form Condensates and Attenuate Apoptosis in Human Cells.
Veling MT, Nguyen DT, Thadani NN, Oster ME, Rollins NJ, Brock KP, Bethel NP, Lim S, Baker D, Way JC, Marks DS, Chang RL, Silver PA
ACS Synth Biol. 2022 Mar 18;11(3):1292-1302. doi: 10.1021/acssynbio.1c00572. Epub 2022 Feb 18.
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PubMed
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Article
Extremotolerance-Associated (ExTol) Protein Collection
This collection contains human expression constructs for proteins associated with extremotolerance in organisms like tardigrades, brine shrimp, rotifers, and resurrection plants. Gene inserts are codon-optimized for expression in human cells and cloned into both light- and chemically-inducible condensate systems to examine apoptosis and condensate formation.
Please see the bioRxiv preprint (Link opens in a new window) for detailed methods using these plasmids, including the following assays:
- Apoptosis assay
- Light-inducible condensate assay
- Chemically-inducible condensate assay
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185139 | CAHS2 (pBS0249) | For the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185140 | SAHS10_A0A1D1UJR2_RAMVA pSecTag (pBS0250) | For the mammalian expression of the tardigrade protein SAHS1_A0A1D1UJR2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185141 | CAHS7_HYPDU_Boothby pSecTag (pBS0251) | For the mammalian expression of the tardigrade protein CAHS7_HYPDU. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185142 | MAHS_RAMVA_Tanaka pSecTag (pBS0253) | For the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185143 | LEA1_APHAV_Chakrabortee pSecTag (pBS0254) | For the mammalian expression of the nematode (Aphelenchus avenae) protein LEA1_APHAV. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185144 | Dehydrin_Q84N45_9LILI pSecTag (pBS0255) | For the mammalian expression of the xerophyta (Xerophyta zambiana) protein Dehydrin_Q84N45_9LILI. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185145 | A0A1D1UNQ6_RAMVA pSecTag2(pm) (pBS0256) | For the mammalian expression of the tardigrade protein A0A1D1UNQ6_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185146 | B9VQB0_ARTSF pSecTag2(pm) (pBS0257) | For the mammalian expression of the brine shrimp protein B9VQB0_ARTSF. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185147 | CAHS1_PARRC_Boothby pSecTag2(pm) (pBS0258) | For the mammalian expression of the tardigrade protein CAHS1_PARRC. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185148 | Q9Y043_CAEEL pSecTag2(pm) (pBS0265) | For the mammalian expression of the C. elegans protein Q9Y043_CAEEL. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185149 | SAHS4_HYPDU pSecTag2(pm) (pBS0266) | For the mammalian expression of the tardigrade protein SAHS4_HYPDU. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185150 | pST_CAHS1_RAMVA (pBS0267) | For the mammalian expression of the tardigrade protein CAHS1_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185151 | pST_CAHS2_PARRC_98-130 (pBS0268) | For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185152 | pST_CAHS2_PARRC_98-187_noLink (pBS0273) | For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-187_noLink. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185153 | pST_YlHD (pBS0275) | For the mammalian expression of the Yarrowia fungus protein YlHD. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185154 | pST_CAHS2_PARRC_98-187 (pBS0278) | For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-187. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185155 | pST_BnHD (pBS0280) | For the mammalian expression of the rapeseed protein BnHD. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185156 | pST_DR_1172_45-268 (pBS0283) | For the mammalian expression of the Deinococcus radiodurans protein DR_1172_45-268. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185157 | pST_CAHS2_HYPDU (pBS0287) | For the mammalian expression of the tardigrade protein CAHS2_HYPDU. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185158 | pST_DR_1172_48-117 (pBS0288) | For the mammalian expression of the Deinococcus radiodurans protein DR_1172_48-117. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185159 | pST_MAHS_RAMVA_81-236 (pBS0289) | For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185160 | pST_CAHS2_RAMVA (pBS0291) | For the mammalian expression of the tardigrade protein CAHS2_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185161 | pST_CAHS2_RAMVA_94-170 (pBS0295) | For the mammalian expression of the tardigrade protein CAHS2_RAMVA_94-170. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185162 | pST_Baker_TJ_190 (pBS0298) | For the mammalian expression of the synthetic protein Baker_TJ_190. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185163 | pST_DR_1172_48-197_noLink (pBS0300) | For the mammalian expression of the Deinococcus radiodurans protein DR_1172_48-197_noLink. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185164 | pST_DR_B0118 (pBS0303) | For the mammalian expression of the Deinococcus radiodurans protein DR_B0118. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185165 | pST_Q3YMR1_CRAPL (pBS0304) | For the mammalian expression of the resurrection plant (Craterostigma plantagineum) protein Q3YMR1_CRAPL. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185166 | pST_A0A1D1UJP0_RAMVA (pBS0306) | For the mammalian expression of the tardigrade protein A0A1D1UJP0_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185167 | pST_CAHS2_PARRC (pBS0307) | For the mammalian expression of the tardigrade protein CAHS2_PARRC. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185168 | pST_G5EFU3_CAEEL (pBS0308) | For the mammalian expression of the C. elegans protein G5EFU3_CAEEL. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185169 | pST_MAHS_RAMVA (pBS0310) | For the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185170 | pST_DR_1172 (pBS0313) | For the mammalian expression of the Deinococcus radiodurans protein DR_1172. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185171 | pST_H2FLK4_CAEEL (pBS0314) | For the mammalian expression of the C. elegans protein H2FLK4_CAEEL. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185172 | pST_A6YT97_ARTSF (pBS0322) | For the mammalian expression of the brine shrimp protein A6YT97_ARTSF. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185173 | pST_O23764_CRAPL (pBS0323) | For the mammalian expression of the resurrection plant (Craterostigma plantagineum) protein O23764_CRAPL. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185174 | pST_APOE_HUMAN_E2_80-255 (pBS0326) | For the mammalian expression of the human protein APOE_HUMAN_E2_80-255. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185175 | pST_B9VQB0_ARTSF (pBS0327) | For the mammalian expression of the brine shrimp protein B9VQB0_ARTSF. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185176 | pST_B1PM76_ARTSF (pBS0329) | For the mammalian expression of the brine shrimp protein B1PM76_ARTSF. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185177 | pST_SYUA_HUMAN (pBS0330) | For the mammalian expression of the human protein SYUA_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185178 | pST_CAHS6_HYPDU (pBS0331) | For the mammalian expression of the tardigrade protein CAHS6_HYPDU. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185179 | pST_CAHS3_HYPDU (pBS0333) | For the mammalian expression of the tardigrade protein CAHS3_HYPDU. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185180 | pST_LEA1_APHAV (pBS0335) | For the mammalian expression of the nematode (Aphelenchus avenae) protein LEA1_APHAV. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185181 | pST_IGKV_ANDDA_Unigene43915 (pBS0336) | For the mammalian expression of the Chinese giant salamander protein IGKV_ANDDA_Unigene43915. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185182 | pST_H9G2T8_CAEEL (pBS0342) | For the mammalian expression of the C. elegans protein H9G2T8_CAEEL. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185183 | pST_H9G2T9_CAEEL (pBS0344) | For the mammalian expression of the C. elegans protein H9G2T9_CAEEL. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185185 | pST_H9G2U1_CAEEL (pBS0346) | For the mammalian expression of the C. elegans protein H9G2U1_CAEEL. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185186 | pST_B4YSF1_9LAMI (pBS0347) | For the mammalian expression of the Dorcoceras hygrometricum protein B4YSF1_9LAMI. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185187 | pST_A0A061ACN9_CAEEL (pBS0349) | For the mammalian expression of the C. elegans protein A0A061ACN9_CAEEL. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185188 | pST_A0A1D1UKG0_RAMVA (pBS0352) | For the mammalian expression of the tardigrade protein A0A1D1UKG0_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185189 | pST_DSUP_RAMVA (pBS0353) | For the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185190 | pST_APOA4_HUMAN (pBS0645) | For the mammalian expression of the human protein APOA4_HUMAN. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185191 | pST_APOE_HUMAN_E3_80-255 (pBS0646) | For the mammalian expression of the human protein APOE_HUMAN_E3_80-255. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185192 | pST_APOE_HUMAN_E4_80-255 (pBS0647) | For the mammalian expression of the human protein APOE_HUMAN_E4_80-255. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185193 | pST_APOE_HUMAN_E3 (pBS0648) | For the mammalian expression of the human protein APOE_HUMAN_E3. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185194 | pST_CAHS1_HYPDU (pBS0650) | For the mammalian expression of the tardigrade protein CAHS1_HYPDU. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185195 | pST_CAHS1_PARRC_101-189 (pBS0651) | For the mammalian expression of the tardigrade protein CAHS1_PARRC_101-189. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185196 | pST_CAHS1_RAMVA_108-197 (pBS0652) | For the mammalian expression of the tardigrade protein CAHS1_RAMVA_108-197. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185197 | pST_CAHS1_PARRC_101-189_noLink (pBS0653) | For the mammalian expression of the tardigrade protein CAHS1_PARRC_101-189_noLink. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185198 | pST_CAHS1_RAMVA_108-197_noLink (pBS0654) | For the mammalian expression of the tardigrade protein CAHS1_RAMVA_108-197_noLink. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185199 | pST_CAHS1_PARRC_101-144 (pBS0656) | For the mammalian expression of the tardigrade protein CAHS1_PARRC_101-144. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185200 | pST_CAHS1_HYPDU_149-191 (pBS0657) | For the mammalian expression of the tardigrade protein CAHS1_HYPDU_149-191. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185201 | pST_CAHS1_PARRC_150-189 (pBS0658) | For the mammalian expression of the tardigrade protein CAHS1_PARRC_150-189. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185202 | pST_A0A1I1U3P5_DrHD_b03_dom3 (pBS0665) | For the mammalian expression of the Deinococcus radiodurans protein A0A1I1U3P5_DrHD_b03_dom3. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185203 | pST_H2FLK7_DrHD_b03_dom1 (pBS0668) | For the mammalian expression of the Deinococcus radiodurans protein H2FLK7_DrHD_b03_dom1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185204 | pST_Q96Q06-2_DrHD_b03_dom1 (pBS0676) | For the mammalian expression of the Deinococcus radiodurans protein Q96Q06-2_DrHD_b03_dom1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185205 | pST_A8E631_DrHD_b03_dom1 (pBS0678) | For the mammalian expression of the Deinococcus radiodurans protein A8E631_DrHD_b03_dom1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185206 | pST_H2FLK7_DrHD_b03_dom3 (pBS0679) | For the mammalian expression of the Deinococcus radiodurans protein H2FLK7_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185207 | pST_D4NWF2_DrHD_b03_dom3 (pBS0681) | For the mammalian expression of the Deinococcus radiodurans protein D4NWF2_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185208 | pST_Q1XI24_DrHD_b03_dom3 (pBS0682) | For the mammalian expression of the Deinococcus radiodurans protein Q1XI24_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185209 | pST_P06727_DrHD_b03_dom3 (pBS0683) | For the mammalian expression of the Deinococcus radiodurans protein P06727_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185210 | pST_UPI0005D02939_DrHD_b03_dom3 (pBS0684) | For the mammalian expression of the Deinococcus radiodurans protein UPI0005D02939_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185211 | pST_Q96Q06-2_DrHD_b03_dom3 (pBS0687) | For the mammalian expression of the Deinococcus radiodurans protein Q96Q06-2_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185212 | pST_DrHD_b03-dom3_opt1 (pBS0692) | For the mammalian expression of the Deinococcus radiodurans protein DrHD_b03-dom3_opt1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185213 | pST_DrHD_b03-dom3_opt2 (pBS0693) | For the mammalian expression of the Deinococcus radiodurans protein DrHD_b03-dom3_opt2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185214 | pST_H2FLK5_CAEEL_542-800 (pBS0695) | For the mammalian expression of the C. elegans protein H2FLK5_CAEEL_542-800. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185215 | pST_H2FLK5_CAEEL_199-435 (pBS0696) | For the mammalian expression of the C. elegans protein H2FLK5_CAEEL_199-435. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185216 | pST_pvLEA22mer_mutant_10 (pBS0705) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185217 | pST_pvLEA22mer_mutant_14 (pBS0709) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_14. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185218 | pST_pvLEA22mer_mutant_16 (pBS0711) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_16. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185219 | pST_pvLEA22mer_mutant_17 (pBS0712) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_17. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185220 | pST_pvLEA22mer_mutant_2 (pBS0715) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_2. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185221 | pST_pvLEA22mer_mutant_21 (pBS0717) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_21. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185222 | pST_pvLEA22mer_mutant_22 (pBS0718) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_22. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185223 | pST_pvLEA22mer_mutant_23 (pBS0719) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_23. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185224 | pST_pvLEA22mer_mutant_3 (pBS0720) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185225 | pST_pvLEA22mer_mutant_5 (pBS0722) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_5. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185226 | pST_pvLEA22mer_shuffle_1 (pBS0727) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185227 | pST_pvLEA22mer_shuffle_3 (pBS0729) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185228 | pST_pvLEA22mer_shuffle_5 (pBS0731) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_5. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185229 | pST_Q19228_CAEEL_51-127_noLink (pBS0733) | For the mammalian expression of the C. elegans protein Q19228_CAEEL_51-127_noLink. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185230 | pST_Q19228_CAEEL_51-88 (pBS0734) | For the mammalian expression of the C. elegans protein Q19228_CAEEL_51-88. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185231 | pST_Q19228_CAEEL_93-127 (pBS0735) | For the mammalian expression of the C. elegans protein Q19228_CAEEL_93-127. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185232 | pST_Q7JLY3_CAEEL_48-124 (pBS0738) | For the mammalian expression of the C. elegans protein Q7JLY3_CAEEL_48-124. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185233 | pST_AfLEA1.1 (pBS0741) | For the mammalian expression of the brine shrimp protein AfLEA1.1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185234 | pST_APOA4_ANDDA_21-367 (pBS0742) | For the mammalian expression of the Chinese giant salamander protein APOA4_ANDDA_21-367. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185235 | pST_APOA4_ANDDA_52-358 (pBS0744) | For the mammalian expression of the Chinese giant salamander protein APOA4_ANDDA_52-358. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185236 | pST_AdAPOA4-44 (pBS0745) | For the mammalian expression of the Chinese giant salamander protein AdAPOA4-44. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185237 | pST_AdHsAPO-44 (pBS0746) | For the mammalian expression of the Chinese giant salamander and human fusion protein AdHsAPO-44. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185238 | pST_CI016_HUMAN (pBS0747) | For the mammalian expression of the human protein CI016_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185239 | pST_SERF2_HUMAN (pBS0751) | For the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185240 | pST_DrHD_dom3_k3_1 (pBS0754) | For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185241 | pST_DrHD_dom3_k3_7 (pBS0755) | For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_7. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185242 | pST_DrHD_dom3_k5_0 (pBS0758) | For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k5_0. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185243 | pST_PvLEA4_repeats_k3_11 (pBS0761) | For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_11. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185244 | pST_PvLEA4_repeats_k3_26 (pBS0762) | For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185245 | pST_PvLEA4_repeats_k4_1 (pBS0763) | For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k4_1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185246 | pST_PvLEA4_repeats_k4_14 (pBS0764) | For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k4_14. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185247 | pST_PvLEA4_repeats_k5_1 (pBS0766) | For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185248 | pST_PvLEA4_repeats_k5_15 (pBS0767) | For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_15. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185249 | pST_SAHS4_k1_0 (pBS0769) | For the mammalian expression of the tardigrade protein SAHS4_k1_0. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185250 | pST_TJ190_negative (pBS0773) | For the mammalian expression of the synthetic protein TJ190_negative. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185251 | pST_DHR93_NCcapped (pBS0777) | For the mammalian expression of the synthetic protein DHR93_NCcapped. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185252 | pST_neg_DHR93 (pBS0779) | For the mammalian expression of the synthetic protein neg_DHR93. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185253 | pST_DHN_AmDHN132 (pBS0781) | For the mammalian expression of the Ammopiptanthus protein DHN_AmDHN132. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185254 | pST_DHN_AtCor47 (pBS0784) | For the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185255 | pST_DHN_CsLEA11 (pBS0786) | For the mammalian expression of the Cucumber protein DHN_CsLEA11. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185256 | pST_DHN_HbDHN1 (pBS0788) | For the mammalian expression of the Para rubber tree protein DHN_HbDHN1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185257 | pST_DHN_HbDHN2 (pBS0789) | For the mammalian expression of the Para rubber tree protein DHN_HbDHN2. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185258 | pST_DHN_MdoDHN11 (pBS0790) | For the mammalian expression of the apple tree protein DHN_MdoDHN11. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185259 | pST_DHN_OsLEA5 (pBS0793) | For the mammalian expression of the rice plant protein DHN_OsLEA5. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185260 | pST_DHN_ShDHN (pBS0797) | For the mammalian expression of the tomato plant protein DHN_ShDHN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185261 | pST_DHN_SpDHN1 (pBS0799) | For the mammalian expression of the grass (Stipa purpurea) protein DHN_SpDHN1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185262 | pST_DHN_TaDHN5 (pBS0801) | For the mammalian expression of the durm wheat protein DHN_TaDHN5. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185263 | pST_DHN_TaLEA4 (pBS0802) | For the mammalian expression of the common wheat protein DHN_TaLEA4. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185264 | pST_DHN_TsDHN-1 (pBS0805) | For the mammalian expression of the Eutrema salsugineum protein DHN_TsDHN-1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185265 | pST_DHN_TsDHN-2 (pBS0806) | For the mammalian expression of the Eutrema salsugineum protein DHN_TsDHN-2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185266 | pST_DHN_VrDHN1a (or YSK2) (pBS0807) | For the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a (or YSK2). This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185267 | pST_APOE_HUMAN_A234G (pBS0816) | For the mammalian expression of the human protein APOE_HUMAN_A234G. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185268 | pST_APOE_HUMAN_T243A (pBS0818) | For the mammalian expression of the human protein APOE_HUMAN_T243A. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185269 | pST_APOE_HUMAN_C130K (pBS0819) | For the mammalian expression of the human protein APOE_HUMAN_C130K. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185270 | pST_APOE_HUMAN_V153N (pBS0824) | For the mammalian expression of the human protein APOE_HUMAN_V153N. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185271 | pST_APOE_HUMAN_R160W (pBS0835) | For the mammalian expression of the human protein APOE_HUMAN_R160W. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185272 | pST_APOE_HUMAN_Y136C (pBS0836) | For the mammalian expression of the human protein APOE_HUMAN_Y136C. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185273 | pST_APOE_HUMAN_E114C (pBS0838) | For the mammalian expression of the human protein APOE_HUMAN_E114C. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185274 | pST_APOE_HUMAN_E114I (pBS0839) | For the mammalian expression of the human protein APOE_HUMAN_E114I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185275 | pST_APOE_HUMAN_D125I (pBS0840) | For the mammalian expression of the human protein APOE_HUMAN_D125I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185276 | pST_APOE_HUMAN_R163C (pBS0842) | For the mammalian expression of the human protein APOE_HUMAN_R163C. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185277 | pST_APOE_HUMAN_K164E (pBS0843) | For the mammalian expression of the human protein APOE_HUMAN_K164E. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185278 | pST_APOE_HUMAN_E2_47-300 (pBS0846) | For the mammalian expression of the human protein APOE_HUMAN_E2_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185279 | pST_APOE_HUMAN_E4_47-300 (pBS0847) | For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185280 | pST_APOE_HUMAN_E2_255-317 (pBS0848) | For the mammalian expression of the human protein APOE_HUMAN_E2_255-317. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185281 | pST_APOE_HUMAN_V153A_G131S_11s (pBS0850) | For the mammalian expression of the human protein APOE_HUMAN_V153A_G131S_11s. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185282 | XIAP (pBS0852) | For the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay. |
| 185283 | pST_H2FLK5_CAEEL_30-800 (pBS0864) | For the mammalian expression of the synthetic protein H2FLK5_CAEEL_30-800. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185284 | pST_AfrLEA2 (pBS0910) | For the mammalian expression of the brine shrimp protein AfrLEA2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185285 | pST_AfrLEA3m (pBS0911) | For the mammalian expression of the brine shrimp protein AfrLEA3m. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185286 | pST_MAHS_RAMVA_mts_1-74 (pBS0917) | For the mammalian expression of the tardigrade protein MAHS_RAMVA_mts_1-74. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185287 | pST_MAHS_RAMVA_mts_1-81 (pBS0918) | For the mammalian expression of the tardigrade protein MAHS_RAMVA_mts_1-81. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). |
| 185288 | LLPS1-FUSN:SspB (pBS1041) | For the mammalian expression of the human protein FUSN attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185289 | LLPS1-iLID::EGFP::FTH1 (pBS1042) | For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185290 | LLPS2-CAHS2_PARRC::SspB (pBS1043) | For the mammalian expression of the tardigrade protein CAHS2_PARRC attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185291 | ApoE2_47-300_mCh-SspB (pBS1068) | For the mammalian expression of the human protein ApoE2_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185292 | ApoE4_47-300_mCh-SspB (pBS1069) | For the mammalian expression of the human protein ApoE4_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185293 | ApoE3_47-300_mCh-SspB (pBS1070) | For the mammalian expression of the human protein ApoE3_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185294 | ApoE3_D125I_47-300_mCh-SspB (pBS1071) | For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185295 | DHR93_Nccapped_mCh-SspB (pBS1072) | For the mammalian expression of the synthetic protein DHR93_Nccapped attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185296 | DSUP_RAMVA_1-208_mCh-SspB (pBS1073) | For the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185298 | DHN_VrDHN1a_mCh-SspB (pBS1074) | Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation. |
| 185299 | PvLEA4_repeats_k3_26_mCh-SspB (pBS1075) | Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation. |
| 185300 | CAHS2_PARRC_98-130_mCh-SspB (pBS1077) | For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185301 | pvLEA22mer_mutant_10_mCh-SspB (pBS1078) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185302 | PvLEA4_repeats_k5_1_mCh-SspB (pBS1079) | For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185303 | pvLEA22mer_mutant_3_mCh-SspB (pBS1080) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185304 | CAHS2parrc-mCh-2xFKBP (pBS1109) | For the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185305 | CAHS3hypdu-mCh-2xFKBP (pBS1110) | For the mammalian expression of the tardigrade protein CAHS3hypdu attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185306 | ApoE2_47-300_mCh-2xFKBP (pBS1111) | For the mammalian expression of the human protein ApoE2_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185307 | ApoE4_47-300_mCh-2xFKBP (pBS1112) | For the mammalian expression of the human protein ApoE4_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185308 | ApoE3_47-300_mCh-2xFKBP (pBS1113) | For the mammalian expression of the human protein ApoE3_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185309 | ApoE3_D125I_47-300_mCh-2xFKBP (pBS1114) | For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185310 | DHR93_Nccapped_mCh-2xFKBP (pBS1115) | For the mammalian expression of the synthetic protein DHR93_Nccapped attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185311 | DSUP_RAMVA_1-208_mCh-2xFKBP (pBS1116) | For the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185312 | DHN_VrDHN1a_mCh-2xFKBP (pBS1117) | For the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185313 | PvLEA4_repeats_k3_26_mCh-2xFKBP (pBS1118) | For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185314 | pvLEA22mer_shuffle_3_mCh-2xFKBP (pBS1119) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185315 | CAHS2_PARRC_98-130_mCh-2xFKBP (pBS1120) | For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185316 | pvLEA22mer_mutant_10_mCh-2xFKBP (pBS1121) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185317 | PvLEA4_repeats_k5_1_mCh-2xFKBP (pBS1122) | For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185318 | pvLEA22mer_mutant_3_mCh-2xFKBP (pBS1123) | For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185319 | SYUA_HUMAN_mCh-2xFKBP (pBS1135) | For the mammalian expression of the human protein SYUA_HUMAN attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185320 | CAHS1_PARRC_101-189_mCh-2xFKBP (pBS1136) | For the mammalian expression of the tardigrade protein CAHS1_PARRC_101-189 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185321 | AfrLEA3m_mCh-2xFKBP (pBS1137) | For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185322 | mCh-2xFKBP (no insert) (pBS1139) | The empty vector used for the FKBP experiments in our paper |
| 185323 | empty-mCh-SspB (pBS1140) | The empty vector used for the iLID-SspB experiments in our paper |
| 185324 | ApoE2_mCh-SspB (pBS1142) | For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185325 | ApoE4_mCh-SspB (pBS1143) | For the mammalian expression of the human protein ApoE4 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185326 | ApoE3_mCh-SspB (pBS1144) | For the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185327 | ApoE3_D125I_mCh-SspB (pBS1145) | For the mammalian expression of the human protein ApoE3_D125I attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation |
| 185328 | pST_MAHS_RAMVA_81-236-FLAG (pBS0297) | For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). |
| 185329 | ApoE3_mCh-2xFKBP (pBS1156) | For the mammalian expression of the human protein ApoE3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |
| 185330 | ApoE3_D125I_mCh-2xFKBP (pBS1157) | For the mammalian expression of the human protein ApoE3_D125I attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation |