Characterization of nuclear pore complex targeting domains in Pom152 in Saccharomyces cerevisiae.
Brown JT, Haraczy AJ, Wilhelm CM, Belanger KD
Biol Open. 2021 Oct 15;10(10). pii: 272274. doi: 10.1242/bio.057661. Epub 2021 Oct 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185097 | KBB464 | HA-tagged POM152 with glycosylation sites mutated and with GFP at C-terminus |
| 185102 | KBB520 | 1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP |
| 185103 | KBB521 | 1st 960 amino acids (C-terminal truncation) of Pom152 fused with HA and GFP |
| 185107 | KBB553 | 1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with Y180L, Q182L |
| 185108 | KBB556 | 1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with S158A, F159A, F160A |