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Exportin Crm1 is important for Swi6 nuclear shuttling and MBF transcription activation in Saccharomyces cerevisiae.

Belanger KD, Yewdell WT, Barber MF, Russo AN, Pettit MA, Damuth EK, Hussain N, Geier SJ, Belanger KG
BMC Mol Cell Biol. 2022 Feb 21;23(1):10. doi: 10.1186/s12860-022-00409-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
185081KBB353Swi6M-GFP expressing N-term 181 amino acids of Swi6
185082KBB354Swi6L-GFP expressin N-term 273 amino acids of Swi6
185087KBB416Swi6L-GFP with nuclear export signal mutated to alanines
185088KBB418Swi6L-GFP with nuclear export signal mutated to alanines
185089KBB419Swi6L-GFP expressing N-term 273 amino acids of Swi6
185131KBB441Swi6 full length with L->A mutated in nuclear localization signal
185132KBB484SWI6-GFP full length under control of GAL1 promotion
185133KBB486Full length CRM1 fused to binding domain of two-hybrid plasmid
185134KBB491Full length SWI6 fused to activation domain of two-hybrid plasmid

Antibodies from Article