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The karyopherin Kap95 and the C-termini of Rfa1, Rfa2, and Rfa3 are necessary for efficient nuclear import of functional RPA complex proteins in Saccharomyces cerevisiae.

Belanger KD, Griffith AL, Baker HL, Hansen JN, Kovacs LA, Seconi JS, Strine AC
DNA Cell Biol. 2011 Sep;30(9):641-51. doi: 10.1089/dna.2010.1071. Epub 2011 Feb 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
185078KBB345rfa3D46 truncation fused to GFP
185080KBB349rfa2D248 truncation fused to GFP
185083KBB364RFA1 fused to GFP under control of GAL1 promoter
185084KBB372GAL::Rfa1[330-621]-GFP under control of GAL1 promoter
185092KBB438GAL1::Rfa1[1-337, 344-621]-GFP with NLS removed

Antibodies from Article