Nuclear localisation is crucial for the proapoptotic activity of the HtrA-like serine protease Nma111p.
Belanger KD, Walter D, Henderson TA, Yelton AL, O'Brien TG, Belanger KG, Geier SJ, Fahrenkrog B
J Cell Sci. 2009 Nov 1;122(Pt 21):3931-41. doi: 10.1242/jcs.056887. Epub 2009 Oct 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185073 | KBB280 | Larger fusion of Nma111 nuclear localization signal to GFP |
| 185074 | KBB282 | Smaller fusion of Nma111 nuclear localization signal to GFP |
| 185086 | KBB394 | NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280) |
| 185090 | KBB428 | NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA) |
| 185091 | KBB430 | NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA) |
| 185093 | KBB439 | NMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion) |
| 185094 | KBB460 | NMA111-GFP wild type under endogenous promoter |
| 185095 | KBB462 | NMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 promoter |
| 185098 | KBB465 | NMA111-GFP with NLS1 deleted under endogenous promoter |
| 185099 | KBB466 | NMA111-GFP with NLS1 and NLS2 deleted under endogenous promoter |
| 185100 | KBB467 | NMA111-GFP with NLS2 deleted under endogenous promoter |
| 185426 | LDB204 | Bacterial expression of FG-repeat domain of NUP1 nucleoporin as GST-Nup1-Rep fusion |