Using affinity chromatography to investigate novel protein-protein interactions in an undergraduate cell and molecular biology lab course.
Belanger KD
CBE Life Sci Educ. 2009 Fall;8(3):214-25. doi: 10.1187/cbe.09-03-0019.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185064 | KBB101 | Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after the last FXFG repeat |
| 185065 | KBB106 | Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion including C-terminal half of Nup1 C-terminal FG domain |
| 185424 | LDB203 | Bacterial expression of N-terminus of NUP1 nucleoporin as GST-Nup1-N fusion |
| 185425 | LDB205 | Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion |
| 185427 | LDB221 | Bacterial expression of Nup1 C-terminal residues 777-1076 as GST fusion |