Oligomerization of p62 allows for selection of ubiquitinated cargo and isolation membrane during selective autophagy.
Wurzer B, Zaffagnini G, Fracchiolla D, Turco E, Abert C, Romanov J, Martens S
Elife. 2015 Sep 28;4:e08941. doi: 10.7554/eLife.08941.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187981 | mCherry-p62-LIR-mutant (LIRAAAA) | Plasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528 |
| 187982 | mcherry-p62 Double Mutation/Silent | K7A/D69A p62 double mutant (PB1AA). Internal reference: SMC532 |
| 187984 | mcherry p62 delta UBA silent | mcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572 |
| 187985 | mcherry-p62 delta PB1 silent | human p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552 |
| 187986 | mCherry-p62-Silent_Mutation_WT | human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516 |
| 199779 | pGEX-4T1_4xUb | Used for the expression of 4X human ubiquitin in tandem (SMC interal no. 538) |
| 199780 | pET-Duet1-His-TEV-mCherry-p62 | Used for the expression of mCherry labelled p62 (SMC Internal No. 391). |