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Hijacking of transcriptional condensates by endogenous retroviruses.

Asimi V, Sampath Kumar A, Niskanen H, Riemenschneider C, Hetzel S, Naderi J, Fasching N, Popitsch N, Du M, Kretzmer H, Smith ZD, Weigert R, Walther M, Mamde S, Meierhofer D, Wittler L, Buschow R, Timmermann B, Cisse II, Ameres SL, Meissner A, Hnisz D
Nat Genet. 2022 Aug;54(8):1238-1247. doi: 10.1038/s41588-022-01132-w. Epub 2022 Jul 21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
185002pX458-IAP-del-gRNA1expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP
185003pX458-IAP-del-gRNA2expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP
185004pX458-MMERVK9C-del-gRNA1expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP
185005pX458-MMERVK9C-del-gRNA2expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP
185006pX458-MMETn-del-gRNA1expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP
185007pX458-MMETn-del-gRNA2expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP
185008pUC19.TRIM28.FKBP.knock-in.mRuby2For knocking in FKBP F36V P2A mRuby2
185009pUC19.TRIM28.FKBP.knock-in.mCeruleanFor knocking in FKBP F36V P2A mCerulean
185010pX458-GFP-TRIM28Encodes gRNA for mouse TRIM28 along with Cas9 with 2A-EGFP
185011pET-45b-POLR2A-CTD-mCherryBacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein
185012pET-45b-HP1a-mCherryBacterial Expression of 6xHis CBX5 (HP1a) mCherry Fusion Protein
185013pET-45b-mEGFPBacterial Expression of 6xHis Sirius-GFP Fusion Protein
185015pET-45b-NFYC-IDR-mEGFPBacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion Protein
185016pLKO.1-Tet-Puro-shIAPEz_1shRNA mediated knockdown
185017pLKO.1-Tet-Puro-shIAPEz_2shRNA mediated knockdown
185018pLKO.1-Tet-Puro-shMMETn_1shRNA mediated knockdown
185019pLKO.1-Tet-Puro-shMMETn_2shRNA mediated knockdown
185020pLKO.1-Tet-Puro-shMMERVK10c_1shRNA mediated knockdown
185021pLKO.1-Tet-Puro-shMMERVK10c_2shRNA mediated knockdown
185022pLKO.1-Tet-Puro-shMMERVK9c_1shRNA mediated knockdown
185023pLKO.1-Tet-Puro-shMMERVK9c_2shRNA mediated knockdown
189953pLKO.1-Tet-Puro-shMMETn_1 (inducible)shRNA mediated knockdown
189954pLKO.1-Tet-Puro-shMMETn_2 (inducible)shRNA mediated knockdown
189955pLKO.1-Tet-Puro-shMMERVK10c_1 (inducible)shRNA mediated knockdown
189956pLKO.1-Tet-Puro-shMMERVK10c_2 (inducible)shRNA mediated knockdown
189957pLKO.1-Tet-Puro-shMMERVK9c_1 (inducible)shRNA mediated knockdown
189958pLKO.1-Tet-Puro-shMMERVK9c_2 (inducible)shRNA mediated knockdown

Antibodies from Article