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Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome.

Durrant MG, Fanton A, Tycko J, Hinks M, Chandrasekaran SS, Perry NT, Schaepe J, Du PP, Lotfy P, Bassik MC, Bintu L, Bhatt AS, Hsu PD
Nat Biotechnol. 2022 Oct 10. pii: 10.1038/s41587-022-01494-w. doi: 10.1038/s41587-022-01494-w. (Link opens in a new window) PubMed (Link opens in a new window) Article


Plasmids from this article have been deposited by multiple labs. See below for all materials from each lab:

  • Michael Bassik
  • Ami Bhatt
  • Lacra Bintu
  • Patrick Hsu
  • Plasmids from Article

    ID Plasmid Purpose
    184944pJT301 BxBi Lenti pEF-attB LSR GFPLentiviral delivery of recombinase landing pad
    184945pJT302 Ec03 Lenti pEF-attB LSR GFPLentiviral delivery of recombinase landing pad
    184946pJT303 Ec04 Lenti pEF-attB LSR GFPLentiviral delivery of recombinase landing pad
    184947pJT304 Ec07 Lenti pEF-attB LSR GFPLentiviral delivery of recombinase landing pad
    184948pJT305 Pa01 Lenti pEF-attB LSR GFPLentiviral delivery of recombinase landing pad
    184949pJT306 Kp03 Lenti pEF-attB LSR GFPLentiviral delivery of recombinase landing pad
    184951pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donorDonor for both PiggyBac and Cp36 Recombinase
    184952pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donorDonor for both PiggyBac and Cp36 Recombinase
    193459pAF0432 Ef1a-Dn29-2a-EGFPExpression of Recombinase
    193460pAF0033 Ef1a-Pa01-2a-EGFPExpression of Recombinase
    193461pAF0034 Ef1a-Kp03-2a-EGFPExpression of Recombinase
    193462pAF0084 Ef1a-Cp36-2a-EGFPExpression of Recombinase
    193463pAF0036 Ef1a-Si74-2a-EGFPExpression of Recombinase
    193464pAF0035 Ef1a-Nm60-2a-EGFPExpression of Recombinase
    193465pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherryDn29 Donor with promoter for mcherry and puro expression
    193466pAF0687 Pa01_attP-mCherry donorPromoterless mcherry donor for Pa01
    193467pAF0688 Kp03_attP-mCherry donorPromoterless mcherry donor for Kp03
    193468pAF0185 Cp36_attP-mCherry donorPromoterless mcherry donor for Cp36
    193469pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donorCp36 Donor with promoter for mcherry and puro expression
    193470pAF0451 Si74_attP-mCherry donorPromoterless mcherry donor for Si74
    193471pAF0443 Nm60_attP-mCherry donorPromoterless mcherry donor for Nm60
    193472pAF0689 Ef1a-Pa01_attB-BFP acceptorAcceptor plasmid for 3 plasmid recombination assay
    193473pAF0690 Ef1a-Kp03_attB-BFP acceptorAcceptor plasmid for 3 plasmid recombination assay
    193474pAF0286 Ef1a-Cp36_attB-BFP acceptorAcceptor plasmid for 3 plasmid recombination assay
    193475pAF0474 Ef1a-Si74_attB acceptorAcceptor plasmid for 3 plasmid recombination assay
    193476pAF0466 Ef1a-Nm60_attB acceptorAcceptor plasmid for 3 plasmid recombination assay

    Antibodies from Article