Precise genome editing across kingdoms of life using retron-derived DNA.
Lopez SC, Crawford KD, Lear SK, Bhattarai-Kline S, Shipman SL
Nat Chem Biol. 2022 Feb;18(2):199-206. doi: 10.1038/s41589-021-00927-y. Epub 2021 Dec 23.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184953 | pSLS.436 | Express -Eco1 RT and ncRNA |
| 184955 | pSLS.601 | Express -Eco1 ncRNA library variants |
| 184956 | pSLS.491 | Test effect of a1/a2 length on editing rpoB using retron recombineering |
| 184957 | pSLS.492 | Test effect of a1/a2 length on editing rpoB using retron recombineering |
| 184958 | pSLS.539 | Test effect of a1/a2 length on editing fabH using retron recombineering |
| 184959 | pSLS.540 | Test effect of a1/a2 length on editing fabH using retron recombineering |
| 184960 | pSLS.543 | Test effect of a1/a2 length on editing murF using retron recombineering |
| 184961 | pSLS.544 | Test effect of a1/a2 length on editing murF using retron recombineering |
| 184962 | pSLS.545 | Test effect of a1/a2 length on editing priB using retron recombineering |
| 184963 | pSLS.546 | Test effect of a1/a2 length on editing priB using retron recombineering |
| 184964 | pSCL.027 | Express -Eco1 RT and ncRNA in yeast |
| 184965 | pSCL.028 | Test effect of extending -Eco1 a1/a2 in yeast |
| 184966 | pSCL.037 | Express -Eco1 dead RT and ncRNA in yeasst |
| 184968 | pSCL.031 | Test effect of extending -Eco2 a1/a2 in yeast |
| 184969 | pSCL.004 | Negative control, empty integrating inducible casette |
| 184970 | pSCL.006 | Express -Eco1 RT in yeast |
| 184971 | pSCL.005 | Express Cas9 in yeast |
| 184972 | pSCL.002 | Express -Eco1 ADE2 editing ncRNA and gRNA |
| 184973 | pSCL.039 | Test effect of extending a1/a2 on ADE2 editing rates in yeast |
| 184974 | pSCL.040 | Test effect of extending a1/a2 on ADE2 editing rates in yeast |
| 184975 | pSCL.106 | Express -Eco1 CAN1 editing ncRNA and gRNA |
| 184976 | pSCL.107 | Test effect of extending a1/a2 on CAN1 editing rates in yeast |
| 184977 | pSCL.108 | Express -Eco1 LYP1 editing ncRNA and gRNA |
| 184978 | pSCL.109 | Test effect of extending a1/a2 on LYP1 editing rates in yeast |
| 184979 | pSCL.110 | Express -Eco1 TRP2 editing ncRNA and gRNA |
| 184980 | pSCL.111 | Test effect of extending a1/a2 on TRP2 editing rates in yeast |
| 184981 | pSCL.112 | Express -Eco1 FAA1 editing ncRNA and gRNA |
| 184982 | pSCL.113 | Test effect of extending a1/a2 on FAA1 editing rates in yeast |
| 184983 | pSCL.71 | Express -Eco1 RT/Cas9 fusion, using linker1 |
| 184984 | pSCL.72 | Express Cas9/-Eco1 RT fusion, using linker1 |
| 184985 | pSCL.94 | Express -Eco1 RT/Cas9 fusion, using linker2 |
| 184986 | pSCL.95 | Express Cas9/-Eco1 RT fusion, using linker2 |
| 184987 | pSCL.102 | Express -Eco1 RT-P2A-Cas9 |
| 184988 | pSCL.103 | Express Cas9-P2A-Eco1RT |
| 184992 | pKDC.015 | Express -Eco2 RT and ncRNA in human cells |
| 184994 | pSCL.139 | Express Cas9-P2A-Eco1RT in human cells |
| 184995 | pSCL.140 | Express Cas9 in human cells |
| 184996 | pSCL.175 | Express -Eco1 HEK3 editing ncRNA and gRNA |
| 184997 | pSCL.176 | Express -Eco1 RNF2 editing ncRNA and gRNA |
| 184998 | pSCL.177 | Express -Eco1 EMX1 editing ncRNA and gRNA |
| 184999 | pSCL.178 | Express -Eco1 FANCF editing ncRNA and gRNA |
| 185000 | pSCL.179 | Express -Eco1 HEK4 editing ncRNA and gRNA |
| 185001 | pSCL.180 | Express -Eco1 AAVS1 editing ncRNA and gRNA |
| 191530 | bSLS.114 | Contains a knockout gene in the E. coli strain BL21-AI |
| 220588 | bMS.346 | Increase editing efficiency of retron and oligo recombineering |