A multifunctional system for genome editing and large-scale interspecies gene transfer.

Teufel M, Klein CA, Mager M, Sobetzko P
Nat Commun. 2022 Jun 14;13(1):3430. doi: 10.1038/s41467-022-30843-1. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
184860	cr3Ec	cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system
184861	cr3Vn	cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination system
184862	cr3Dd	cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system
184863	pSWAP_Entry_T1	pSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.
184864	pSWAP_Entry_T2	pSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.
184865	pSWAP_Entry_H1_alpha	pSwap plasmid part with H1_alpha homology arms
184866	pSWAP_Entry_HA	pSwap plasmid part with HA homology arms
184867	pSWAP_Entry_INS	pSwap plasmid part with insert fragment module
184868	pSWAP_Entry_HB	pSwap plasmid part with HB homology arms
184869	pSWAP_Entry_HAIB	For scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules.
184870	pSWAP_Entry_H2_beta_CM	pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette
184871	pSWAP_Entry_H2_beta_GM	pSwap plasmid part with H2_beta homology arms and gentamycin cassette
184872	pDrop	Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.
184873	pDropVn	Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.