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A Method for In Situ Reverse Genetic Analysis of Proteins Involved mtDNA Replication.

Kozhukhar N, Spadafora D, Rodriguez YAR, Alexeyev MF
Cells. 2022 Jul 11;11(14). pii: cells11142168. doi: 10.3390/cells11142168. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
184848pMA3735gRNA cloning using blue-white screening for recombinants
184849pMA4854Coexpression of PhiC31o recombinase and EGFP
184850pMA5096General purpose retroviral cloning vector, hygromycin resistance
184851pMA3998rcA retroviral vector with blasticidin resistance for cloning inserts between loxP sites
184852pMA3442Retroviral vector encoding puromycin resistance and Cre recombinase
184853pMA5136A retroviral vector encoding hygromycin resistance and PhiC31 recombinase
184854pMA4659A retroviral vector with G418 resistance for cloning inserts between PhiC31 attP and attB sites
184855pMA5460Encodes G418 resistance and PhiC31-excisable hTFAM gene
184857pMA4000Cre-excisable TFAM in Blasticidin-resistannce retroviral vector
184858pMA5132Retroviral vector, hygromycin resistance hTFAM expression

Antibodies from Article