Genetically defined nucleus incertus neurons differ in connectivity and function
Spikol ED, Cheng J, Macurak M, Subedi A, Halpern ME
eLife 12:RP89516
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184811 | pDestTol2-QUAS:GFP | Used to generate the Tg(QUAS:GFP)c578 transgenic line |
| 184812 | pDestTol2-QUAS:mApple-he1.1:CFP | Used to generate the Tg(QUAS:mApple; he1.1:CFP)c788 transgenic line |
| 184813 | pDestTol2-QUAS:GFP-CAAX | Used to generate the Tg(QUAS:GFP-CAAX)c591 transgenic line |
| 184814 | pDestTol2-QUAS:NLS-mApple-he1.1:CFP | Used to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic line |
| 184815 | pDestTol2-QUAS:NLS-GFP-he1.1:CFP | Used to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic line |
| 184816 | pDestTol2-QUAS:GFP-CAAX-he1.1:YFP | Used to generate the Tg(QUAS:GFP-CAAX; he1.1:YFP)c631 transgenic line |
| 184817 | pDestTol2-QUAS:GCaMP7a | Used to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040). |
| 184818 | pDR274-gsc2-sgRNA | Used to synthesize gRNA targeting exon 2 of the gsc2 gene |
| 184819 | pPRISM-QF2-he1.1:YFP | Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2. |
| 184820 | pPRISM-QF2-he1.1:YFP-rln3a-HA | Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration. |
| 184821 | TOPO-nmba | Used to synthesize RNA probe for in situ hybridization for nmba |
| 184822 | TOPO-nmbb | Used to synthesize RNA probe for in situ hybridization for nmbb |
| 184823 | TOPO-nts | Used to synthesize RNA probe for in situ hybridization for nts |