Friction-driven membrane scission by the human ESCRT-III proteins CHMP1B and IST1.
Cada AK, Pavlin MR, Castillo JP, Tong AB, Larsen KP, Ren X, Yokom AL, Tsai FC, Shiah JV, Bassereau PM, Bustamante CJ, Hurley JH
Proc Natl Acad Sci U S A. 2022 Jul 19;119(29):e2204536119. doi: 10.1073/pnas.2204536119. Epub 2022 Jul 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184803 | His-MBP-CHMP1B, S2C | Bacterial expression of CHMP1B (S2C mutation) |
| 184804 | His-GST-Cys-M87 Spastin | Bacterial expression of M87 Spastin (A2C mutation) |
| 184805 | His-MBP-IST1 N-terminal domain | Bacterial expression of IST1 N-terminal domain |
| 184806 | VPS4B, S2C | Bacterial expression of VPS4B (S2C mutation) |