Grainyhead 1 acts as a drug-inducible conserved transcriptional regulator linked to insulin signaling and lifespan.
Grigolon G, Araldi E, Erni R, Wu JY, Thomas C, La Fortezza M, Laube B, Pohlmann D, Stoffel M, Zarse K, Carreira EM, Ristow M, Fischer F
Nat Commun. 2022 Jan 10;13(1):107. doi: 10.1038/s41467-021-27732-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177761 | pdestMB14_grh-1p-grh-1-gfp | Overexpression of C. elegans grh-1 |
| 177762 | pdestMB14_hsp-16.2p-grh-1-gfp | Overexpression of C. elegans grh-1 |
| 177763 | PX459_GRHL1-exon5 | sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1 |
| 177764 | PX459_GRHL1-exon8 | sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1 |
| 177765 | pcDNA3.1_Hygro(+)_GRHL1-L3F6H | Overexpression of 3xFLAG-6xHis tagged human GRHL1 |
| 177767 | pcDNA3.1_Hygro(+)_GRHL1-R9A-L3F6H | Overexpression of 3xFLAG-6xHis tagged human GRHL1 (R9A mutation) |
| 177768 | pcDNA3.1_Hygro(+)_GRHL1-S95A-L3F6H | Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S95A mutation) |
| 177769 | pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H | Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation) |
| 177770 | pcDNA3.1_Hygro(+)_GRHL1-T139A-L3F6H | Overexpression of 3xFLAG-6xHis tagged human GRHL1 (T139A mutation) |
| 177771 | pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H | Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation) |
| 177772 | pcDNA3.1_Hygro(+)_GRHL1-S374A-L3F6H | Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S374A mutation) |
| 177773 | pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H | Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation) |
| 177774 | pGL4.27_GRHL-SPE | Luciferase reporter for GRHL |
| 177775 | pGL4.27_ARE/NRF2-SPE | Luciferase reporter for ARE/NRF2 |