Excess diacylglycerol at the endoplasmic reticulum disrupts endomembrane homeostasis and autophagy.
Li D, Yang SG, He CW, Zhang ZT, Liang Y, Li H, Zhu J, Su X, Gong Q, Xie Z
BMC Biol. 2020 Aug 28;18(1):107. doi: 10.1186/s12915-020-00837-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184758 | OM14-2EGFP-UG74-ter | Marker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6. |
| 184759 | UG75-DGK1-3HA | DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6. |
| 184760 | UG75-CDS1-3HA | CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6. |
| 184761 | UG75-PAH1-3HA | PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6. |
| 184762 | UG76-pLYS2-GFP-PKCbeta | Marker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis). |
| 184763 | EMC1-2EGFP-ter-NAT | Marker for yeast endoplasmic reticulum (ER). Integration of 2xGFP tag at EMC1 C terminus. Uses antibiotic resistance marker natMX6. |
| 184764 | SEC7-2EGFP-ter-NAT | Marker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6. |
| 184766 | IRE1-2EGFP-ter-kanMX4 | Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4. |
| 184767 | CIT1-2EGFP-ter-URA3 | Marker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans). |
| 184768 | ABF2-2EGFP-ter-URA3 | Marker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans). |
| 184769 | ACH1-2EGFP-ter-URA3 | Marker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans). |
| 184770 | AFG3-2EGFP-ter-URA3 | Marker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans). |
| 184771 | SYS1-2EGFP-ter-URA3 | Marker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans). |
| 184772 | SEC7-2Katushka-ter-Hygromycin | Red marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6. |
| 184773 | PKC1-2GFP-ter-TRP1 | Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis). |
| 184774 | trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT | Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D. |
| 184775 | GAL-Aur1-2GFP-Hygromycin | Yeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6. |
| 184776 | pTPI1-EMC1-GFP-APEX2-URA3 | Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis). |
| 198595 | UG75-ADH1-DGK1-3HA | DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6. |
| 198596 | UG75-ADH1-CDS1-3HA | CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6. |
| 198597 | UG75-ADH1-PAH1-3HA | PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6. |