Noninvasive assessment of gut function using transcriptional recording sentinel cells.
Schmidt F, Zimmermann J, Tanna T, Farouni R, Conway T, Macpherson AJ, Platt RJ
Science. 2022 May 13;376(6594):eabm6038. doi: 10.1126/science.abm6038. Epub 2022 May 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184738 | pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI_Term_NotI | encodes aTc inducible FsRT-Cas1–Cas2 and transcriptionally insulated FsCRISPR Array 2 |
| 184739 | pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI | encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106 |
| 184740 | pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI | encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109 |
| 184741 | pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI | encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103 |
| 184742 | pFS_1142_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader1-ARRAY1-DR1-FaqI_Term_NotI | encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 1 transcribed by BBa_J23103 |