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Calcium transients trigger switch-like discharge of prostaglandin E(2) in an extracellular signal-regulated kinase-dependent manner.

Watabe T, Yamahira S, Takakura K, Thumkeo D, Narumiya S, Matsuda M, Terai K
eLife. 2024 Jan 26;12:RP86727. doi: 10.7554/eLife.86727. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
184709pPBpuro-4493NES(Booster-PKA)PKA biosensor
184710pCSIIpuro-4493NES(Booster-PKA)PKA biosensor
184711pPBpuro-UbC-4493NES(Booster-PKA)PKA biosensor
184712pCSIIbsr-GCaMP6sCalcium biosensor
184713pCSIIhyg-iRFP670-P2Av3-RGECO1.0Calcium biosensor
184714pPBpuro-hM3D-P2Av3-mCherryNLSGq DREADD
184715pPBbsr2-OptoSTIM1(CRY2clust)OptoSTIM1(CRY2clust)
184716pPBbsr2-EBFP2-OptoSTIM1(CRY2clust)OptoSTIM1(CRY2clust)
184717pPBpuro-miRFP703-eDHFR(69K6)-cRafiRaf
184718pPBbsr2-cPLA2-mEGFPExogenous cPLA2WT
184720pPBpuro-COX2-EBFP2Exogenous COX2
184721pPBbsr2-PTGER2Exogenous EP2
184722LentiCRISPRv2-bleo-COX1-4COX1-KO in MDCK
184723LentiCRISPRv2-hyg2-COX2-4COX2-KO in MDCK
184724LentiCRISPRv2-bleo-PTGER2EP2-KO in MDCK
184725LentiCRISPRv2-bleo-PLA2G4A_3cPLA2-KO in MDCK
184726LentiCRISPRv2-hyg2-PTGER4EP4-KO in MDCK
184727pX459-canis_PTGS1-3COX1-KO in MDCK
184728pX459-canis_PTGS1-4COX1-KO in MDCK
184729pX459-canis_PTGS2-3COX2-KO in MDCK
184730pX459-canis_PTGS2-4COX2-KO in MDCK
184731pX459-homo_PTGS1-1COX1-KO in HeLa
184732pX459-homo_PTGS1-2COX1-KO in HeLa
184733pX459-homo_PTGS2-3COX2-KO in HeLa
184734pX459-homo_PTGS2-4COX2-KO in HeLa
184735pX459-homo_PLA2G4A-1cPLA2-KO in HeLa
184736pX459-homo_PLA2G4A-2cPLA2-KO in HeLa

Antibodies from Article