Targeting stem-loop 1 of the SARS-CoV-2 5' UTR to suppress viral translation and Nsp1 evasion.

Vora SM, Fontana P, Mao T, Leger V, Zhang Y, Fu TM, Lieberman J, Gehrke L, Shi M, Wang L, Iwasaki A, Wu H
Proc Natl Acad Sci U S A. 2022 Mar 1;119(9). pii: 2117198119. doi: 10.1073/pnas.2117198119. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
191485	pLV-mScarlet-SARS-CoV-2-SL1 alone	Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone
191484	pLV-mScarlet-SARS-CoV-2-5'UTRΔSL1	Lentiviral expression of SARS-CoV-2 5' UTR ΔSL1
191483	pCMV-Dest-MBP-Nsp1	Mammalian expression of MBP-tagged SARS-CoV-2 Nsp1
191480	pLV-SARS-CoV-2 5'UTR-Luciferase	Luciferase reporter for SARS-CoV-2 5' UTR
184639	pLV-mScarlet-MAVS-5'UTR	Fluorescent reporter for mammalian cells: Expression of mScarlet with MAVS 5'UTR
184638	pLV-mScarlet-SARS-CoV-2-5'UTR	Fluorescent reporter for mammalian cells: Expression of mScarlet with SARS-CoV-2 5'UTR
184637	pLV-mScarlet-Control-5'UTR	Fluorescent reporter for mammalian cells: Expression of mScarlet with a CMV 5'UTR