PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics.
Acharya S, Ansari AH, Kumar Das P, Hirano S, Aich M, Rauthan R, Mahato S, Maddileti S, Sarkar S, Kumar M, Phutela R, Gulati S, Rahman A, Goel A, Afzal C, Paul D, Agrawal T, Pulimamidi VK, Jalali S, Nishimasu H, Mariappan I, Nureki O, Maiti S, Chakraborty D
Nat Commun. 2024 Jun 28;15(1):5471. doi: 10.1038/s41467-024-49233-w.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201947 | PX458-3xHA-en1FnCas9 | Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA |
| 201948 | PX458-3xHA-en15FnCas9 | Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA |
| 201949 | PX458-3xHA-en31FnCas9 | Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA |
| 201950 | pET-His6-en1dFnCas9GFP | Expression of en1dFnCas9-GFP in bacterial cells |
| 201951 | pET-His6-en15dFnCas9GFP | Expression of en15dFnCas9-GFP in bacterial cells |
| 201952 | PX458-3xHA-SpCas9-HF1 | Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA |
| 201953 | PX458-3xHA-dSpCas9 | Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA |
| 201954 | PX458-3xHA-dFnCas9 | Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA |
| 201955 | PX458-3xHA-FnCas9ABEmax8.17d | Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA |
| 201956 | PX458-3xHA-en31FnCas9ABEmax8.17d | Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA |