Debojyoti Chakraborty Lab:
Engineered PAM-flexible FnCas9 variants with enhanced activity for robust and specific genome editing and diagnostics
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201947 | PX458-3xHA-en1FnCas9 | Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA |
| 201948 | PX458-3xHA-en15FnCas9 | Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA |
| 201949 | PX458-3xHA-en31FnCas9 | Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA |
| 201950 | pET-His6-en1dFnCas9GFP | Expression of en1dFnCas9-GFP in bacterial cells |
| 201951 | pET-His6-en15dFnCas9GFP | Expression of en15dFnCas9-GFP in bacterial cells |
| 201952 | PX458-3xHA-SpCas9-HF1 | Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA |
| 201953 | PX458-3xHA-dSpCas9 | Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA |
| 201954 | PX458-3xHA-dFnCas9 | Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA |
| 201955 | PX458-3xHA-FnCas9ABEmax8.17d | Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA |
| 201956 | PX458-3xHA-en31FnCas9ABEmax8.17d | Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA |