Anti-CRISPR prediction using deep learning reveals an inhibitor of Cas13b nucleases.

Wandera KG, Alkhnbashi OS, Bassett HVI, Mitrofanov A, Hauns S, Migur A, Backofen R, Beisel CL
Mol Cell. 2022 May 24. pii: S1097-2765(22)00437-3. doi: 10.1016/j.molcel.2022.05.003. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
184565	pPbuCas13b	Inducible expression of PbuCas13b nuclease in bacteria.
184566	pAcrVIB1	Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.
184567	pPbuCas13b-gRNA-1	Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
184568	pPbuCas13b-gRNA-NT	Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.
184833	pPgiCas13b	Inducible expression of PgiCas13b nuclease in bacteria.
184834	pPgiCas13b-gRNA-1	Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
184835	pPgiCas13b-gRNA-NT	Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.
184836	pAcrVIB1-cloDF-kan	Expression of anti-CRISPR protein AcrVIB1 in bacteria.
184837	pcloDF-kan	Backbone that confers Kanamycin resistance, contains cloDF ori
184838	pPlacPbuCas13b-gRNA-NT	Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.
184839	pPlacPbuCas13b-gRNA-T-MS2	Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.
184840	pPlacPbuCas13b-gRNA-T-deGFP	Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.
184841	P70a-deGFP-sc101-kan	Expression of deGFP
184842	pPJ23116-MS2-rep	Expression of the rep protein of phage MS2
184843	pAcrVIB1-cloDF-amp	Expression of anti-CRISPR protein AcrVIB1 in bacteria.
184844	pcloDF-amp	Backbone that confers Ampicillin resistance, contains cloDF ori