Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron.
Teng IT, Nazzari AF, Choe M, Liu T, Oliveira de Souza M, Petrova Y, Tsybovsky Y, Wang S, Zhang B, Artamonov M, Madan B, Huang A, Lopez Acevedo SN, Pan X, Ruckwardt TJ, DeKosky BJ, Mascola JR, Misasi J, Sullivan NJ, Zhou T, Kwong PD
PLoS One. 2022 May 24;17(5):e0268767. doi: 10.1371/journal.pone.0268767. eCollection 2022.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176324 | pVRC8400-SARS-CoV-2-S2P-D614G-AVI | Expresses SARS-CoV-2 S2P protein containing D614G mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176325 | pVRC8400-SARS-CoV-2-S2P-B.1.1.7-AVI | Expresses SARS-CoV-2 S2P protein from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176326 | pVRC8400-SARS-CoV-2-S2P-B.1.351-AVI | Expresses SARS-CoV-2 S2P protein from the B.1.351 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176327 | pVRC8400-SARS-CoV-2-S2P-P.1-AVI | Expresses SARS-CoV-2 S2P protein from the P.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176328 | pVRC8400-SARS-CoV-2-S2P-B.1.429-AVI | Expresses SARS-CoV-2 S2P protein from the B.1.429 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176329 | pVRC8400-SARS-CoV-2-S2P-B.1.526-S477N-AVI | Expresses SARS-CoV-2 S2P protein from the B.1.526 variant containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176330 | pVRC8400-SARS-CoV-2-S2P-B.1.526-E484K-AVI | Expresses SARS-CoV-2 S2P protein from the B.1.526 variant containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176331 | pVRC8400-SARS-CoV-2-S2P-B.1.617-E154K-L452R-E484Q-D614G-P681R-AVI | Expresses SARS-CoV-2 S2P protein from the B.1.617 variant containing E154K-L452R-E484Q-D614G-P681R mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176332 | pVRC8400-SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D-AVI | Expresses SARS-CoV-2 S2P protein from the B.1.617 variant containing G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176333 | pVRC8400-SARS-CoV-2-S2P-B.1.617.1-AVI | Expresses SARS-CoV-2 S2P protein from the B.1.617.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176334 | pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVI | Expresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176335 | pVRC8400-SARS-CoV-2-S2P-AY.1-AVI | Expresses SARS-CoV-2 S2P protein from the AY.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176336 | pVRC8400-SARS-CoV-2-S2P-B.1.618-AVI | Expresses SARS-CoV-2 S2P protein from the B.1.618 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176424 | pVRC8400-SARS-CoV-2-NTD-B.1.1.7-AVI | Expresses SARS-CoV-2 NTD domain from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176426 | pVRC8400-SARS-CoV-2-NTD-B.1.351-AVI | Expresses SARS-CoV-2 NTD domain from the B.1.351 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176429 | pVRC8400-SARS-CoV-2-NTD-P.1-AVI | Expresses SARS-CoV-2 NTD domain from the P.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176431 | pVRC8400-SARS-CoV-2-NTD-W152C-AVI | Expresses SARS-CoV-2 NTD domain containing W152C mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176432 | pVRC8400-SARS-CoV-2-NTD-T95I-D253G-AVI | Expresses SARS-CoV-2 NTD domain containing T95I-D253G mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176433 | pVRC8400-SARS-CoV-2-NTD-E154K-AVI | Expresses SARS-CoV-2 NTD domain containing E154K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176434 | pVRC8400-SARS-CoV-2-NTD-G142D-E154K-AVI | Expresses SARS-CoV-2 NTD domain containing G142D-E154K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176435 | pVRC8400-SARS-CoV-2-NTD-B.1.617.1-AVI | Expresses SARS-CoV-2 NTD domain from the B.1.617.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176436 | pVRC8400-SARS-CoV-2-NTD-B.1.617.2-AVI | Expresses SARS-CoV-2 NTD domain from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176437 | pVRC8400-SARS-CoV-2-NTD-AY.1-AVI | Expresses SARS-CoV-2 NTD domain from the AY.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176438 | pVRC8400-SARS-CoV-2-NTD-B.1.618-AVI | Expresses SARS-CoV-2 NTD domain from the B.1.618 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176439 | pVRC8400-SARS-CoV-2-RBD-N501Y-AVI | Expresses SARS-CoV-2 RBD domain containing N501Y mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176440 | pVRC8400-SARS-CoV-2-RBD-K417N-E484K-N501Y-AVI | Expresses SARS-CoV-2 RBD domain containing K417N-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176441 | pVRC8400-SARS-CoV-2-RBD-K417T-E484K-N501Y-AVI | Expresses SARS-CoV-2 RBD domain containing K417T-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176442 | pVRC8400-SARS-CoV-2-RBD-L452R-AVI | Expresses SARS-CoV-2 RBD domain containing L452R mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176443 | pVRC8400-SARS-CoV-2-RBD-S477N-AVI | Expresses SARS-CoV-2 RBD domain containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176444 | pVRC8400-SARS-CoV-2-RBD-E484K-AVI | Expresses SARS-CoV-2 RBD domain containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176445 | pVRC8400-SARS-CoV-2-RBD-L452R-E484Q-AVI | Expresses SARS-CoV-2 RBD domain containing L452R-E484Q mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176446 | pVRC8400-SARS-CoV-2-RBD-L452R-T478K-AVI | Expresses SARS-CoV-2 RBD domain containing L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176447 | pVRC8400-SARS-CoV-2-RBD-K417N-L452R-T478K-AVI | Expresses SARS-CoV-2 RBD domain containing K417N-L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176448 | pVRC8400-SARS-CoV-2-RBD-SD1-WA1-AVI | Expresses SARS-CoV-2 RBD-SD1 domain from the WA1 strain with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176449 | pVRC8400-SARS-CoV-2-RBD-SD1-N501Y-A570D-AVI | Expresses SARS-CoV-2 RBD-SD1 domain containing N501Y-A570D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176450 | pVRC8400-SARS-CoV-2-RBD-SD1-K417N-E484K-N501Y-AVI | Expresses SARS-CoV-2 RBD-SD1 domain containing K417N-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176451 | pVRC8400-SARS-CoV-2-RBD-SD1-K417T-E484K-N501Y-AVI | Expresses SARS-CoV-2 RBD-SD1 domain containing K417T-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176452 | pVRC8400-SARS-CoV-2-RBD-SD1-L452R-AVI | Expresses SARS-CoV-2 RBD-SD1 domain containing L452R mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176453 | pVRC8400-SARS-CoV-2-RBD-SD1-S477N-AVI | Expresses SARS-CoV-2 RBD-SD1 domain containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176454 | pVRC8400-SARS-CoV-2-RBD-SD1-E484K-AVI | Expresses SARS-CoV-2 RBD-SD1 domain containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176455 | pVRC8400-SARS-CoV-2-RBD-SD1-L452R-E484Q-AVI | Expresses SARS-CoV-2 RBD-SD1 domain containing L452R-E484Q mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176456 | pVRC8400-SARS-CoV-2-RBD-SD1-L452R-T478K-AVI | Expresses SARS-CoV-2 RBD-SD1 domain containing L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 176457 | pVRC8400-SARS-CoV-2-RBD-SD1-K417N-L452R-T478K-AVI | Expresses SARS-CoV-2 RBD-SD1 domain containing K417N-L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 181693 | pVRC8400-SARS-CoV-2-S2P-C.37-AVI | Expresses SARS-CoV-2 S2P protein from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 181694 | pVRC8400-SARS-CoV-2-S2P-B.1.621-AVI | Expresses SARS-CoV-2 S2P protein from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 181695 | pVRC8400-SARS-CoV-2-S2P-B.1.1.529-AVI | Expresses SARS-CoV-2 S2P protein from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 181696 | pVRC8400-SARS-CoV-2-NTD-C.37-AVI | Expresses SARS-CoV-2 NTD domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 181697 | pVRC8400-SARS-CoV-2-NTD-B.1.621-AVI | Expresses SARS-CoV-2 NTD domain from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 181698 | pVRC8400-SARS-CoV-2-NTD-B.1.1.529-AVI | Expresses SARS-CoV-2 NTD domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 181699 | pVRC8400-SARS-CoV-2-RBD-C.37-AVI | Expresses SARS-CoV-2 RBD domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 181700 | pVRC8400-SARS-CoV-2-RBD-B.1.621-AVI | Expresses SARS-CoV-2 RBD domain from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 181701 | pVRC8400-SARS-CoV-2-RBD(aa335-526)-B.1.1.529-AVI | Expresses SARS-CoV-2 RBD domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 181702 | pVRC8400-SARS-CoV-2-RBD-SD1-C.37-AVI | Expresses SARS-CoV-2 RBD-SD1 domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 181703 | pVRC8400-SARS-CoV-2-RBD-SD1-B.1.621-AVI | Expresses SARS-CoV-2 RBD-SD1 domain from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 181704 | pVRC8400-SARS-CoV-2-RBD-SD1-B.1.1.529-AVI | Expresses SARS-CoV-2 RBD-SD1 domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 182930 | pVRC8400-SARS-CoV-2-RBD(aa330-526)-B.1.1.529-AVI | Expresses SARS-CoV-2 RBD (aa324-526) domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 184531 | pVRC8400-SARS-CoV-2-S2P-BA.2-AVI | Expresses SARS-CoV-2 S2P protein from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 184532 | pVRC8400-SARS-CoV-2-NTD-BA.2-AVI | Expresses SARS-CoV-2 NTD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 184533 | pVRC8400-SARS-CoV-2-RBD-BA.2-AVI | Expresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 184534 | pVRC8400-SARS-CoV-2-RBD-SD1-BA.2-AVI | Expresses SARS-CoV-2 RBD-SD1 domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 196617 | pVRC8400-SARS-CoV-2-RBD-BJ.1-AVI | Expresses SARS-CoV-2 RBD protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 196618 | pVRC8400-SARS-CoV-2-RBD-BQ.1.1-AVI | Expresses SARS-CoV-2 RBD protein from the BQ.1.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 196619 | pVRC8400-SARS-CoV-2-RBD-XBB-AVI | Expresses SARS-CoV-2 RBD protein from the XBB variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 196620 | pVRC8400-SARS-CoV-2-S2P-BJ.1-AVI | Expresses SARS-CoV-2 S2P protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 196621 | pVRC8400-SARS-CoV-2-S2P-BQ.1.1-AVI | Expresses SARS-CoV-2 S2P protein from the BQ.1.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 196622 | pVRC8400-SARS-CoV-2-S2P-XBB-AVI | Expresses SARS-CoV-2 S2P protein from the XBB variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 198465 | pVRC8400-SARS-CoV-2-S2P-XBB.1.5-AVI | Expresses SARS-CoV-2 S2P protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 198466 | pVRC8400-SARS-CoV-2-RBD-XBB.1.5-AVI | Expresses SARS-CoV-2 RBD protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag |