Next-generation AMA1-based plasmids for enhanced heterologous expression in filamentous fungi.
Roux I, Woodcraft C, Sbaraini N, Pepper A, Wong E, Bracegirdle J, Chooi YH
Microb Biotechnol. 2024 Sep;17(9):e70010. doi: 10.1111/1751-7915.70010.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184497 | pYFAC-ubi-Y-pyrG | AMA1 based vector for optimised episomal expression in filamentous fungi, with increased selection stringency using the modified ubi-Y-pyrG marker (high stringency). |
| 221131 | pYFAC-CH2-ubi-Y-pyrG | Expression of heterologous genes under alcohol induction optimised with ubi-Y-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI. |
| 221132 | pYFAC-CH2-ubi-M-pyrG | Expression of heterologous genes under alcohol induction optimised with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI. |