AGO1 regulates pericentromeric regions in mouse embryonic stem cells
Muller M, Fah T, Schaefer M, Hermes V, Luitz J, Stalder P, Arora R, Ngondo RP, Ciaudo C
Life Sci Alliance. 2022 Mar 2;5(6). pii: 5/6/e202101277. doi: 10.26508/lsa.202101277. Print 2022 Jun.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170916 | pMSCV_PIG_3XHA-Ago1 | Expresses 3X-HA-AGO1 |
| 170917 | pMSCV_Hyg_FLAG-BirA-eGFP-NLS2 | Expresses FLAG-BirA-eGFP-NLS2 to be used as control in BioID or FLAG pull down |
| 170918 | pUC19_HR-BirA-FLAG_Ago2 | Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2 |
| 170919 | pUC19_HR-BirA-FLAG_Ago1 | Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1 |
| 170920 | pX458_sgAgo2_Nterm | Expresses spCas9 and sgRNA targeting the N-terminus of Ago2 for N-terminal tagging |
| 170921 | pX458_sgAgo1_Nterm | Expresses spCas9 and sgRNA targeting the N-terminus of Ago1 for N-terminal tagging |
| 172470 | pX458_sgRNA_Ago1_5 | Expresses SpCas9, GFP, and sgRNA targeting Ago1 |
| 172471 | pX458_sgRNA_Ago1_6 | Expresses SpCas9, GFP, and sgRNA targeting Ago1. |