Activation of stably silenced genes by recruitment of a synthetic de-methylating module.
Chan WF, Coughlan HD, Chen Y, Keenan CR, Smyth GK, Perkins AC, Johanson TM, Allan RS
Nat Commun. 2022 Sep 23;13(1):5582. doi: 10.1038/s41467-022-33181-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184438 | dCas9-5xGCN4-P2A-BFP | dCas9 fused with 5 copies of GCN4 epitope |
| 184439 | scFv-GCN4-sfGFP-TET1CD | TET1 catalytic domain fused with sfGFP and scFv against GCN4 |
| 184440 | MS2-p65-HSF1-P2A-mCherry | MS2 coat protein fused with p65 and hsf1 |
| 184441 | gRNA-MS2x2-TagRFP657 | sgRNA with MS2 aptamer, TagRFP657 as selection marker |
| 184442 | scFv-GCN4-sfGFP-deadTET1CD | Catalytically DEAD TET1CD with scFv against GCN4 |