SNAP- and Halo-tagging and dye introduction protocol for live microscopy in Xenopus embryos.
Dudley CE, van den Goor L, Miller AL
STAR Protoc. 2022 Aug 18;3(3):101622. doi: 10.1016/j.xpro.2022.101622. eCollection 2022 Sep 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184415 | pCS2+/N-SNAPf | vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis |
| 184416 | pCS2+/C-SNAPf | vector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis |
| 184417 | pCS2+/N-Halo | vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis |
| 184418 | pCS2+/C-Halo | vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis |