SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e.
Kulcsar PI, Talas A, Ligeti Z, Krausz SL, Welker E
Nat Commun. 2022 Nov 11;13(1):6858. doi: 10.1038/s41467-022-34527-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184370 | pPIK16045_pX330_Flag-SuperFi-Cas9 | Mammalian expression of SuperFi-Cas9 nuclease |
| 184371 | pAT15541_dCBE-SuperFi-Cas9 | Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor |
| 184372 | pAT15542_nCBE-SuperFi-Cas9 | Mammalian expression of SuperFi-Cas9 CBE base editor |
| 184373 | pAT15543_dABE-SuperFi-Cas9 | Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor |
| 184374 | pAT15544_nABE-SuperFi-Cas9 | Mammalian expression of SuperFi-Cas9 ABE7 base editor |
| 184375 | pAT15545_dABE8e-SuperFi-Cas9 | Mammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editor |
| 184376 | pAT15546_nABE8e-SuperFi-Cas9 | Mammalian expression of SuperFi-Cas9 ABE8e base editor |
| 184377 | pAT15547_PEmax-SuperFi-Cas9 | Mammalian expression of SuperFi-Cas9 PEmax prime editor |