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Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition.

Hauser D, Behr K, Konno K, Schreiner D, Schmidt A, Watanabe M, Bischofberger J, Scheiffele P
Neuron. 2022 May 8. pii: S0896-6273(22)00362-2. doi: 10.1016/j.neuron.2022.04.017. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
184334pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.
184335pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPIExpression of GPI-anchored form of mouse Nrx3-alpha containing the alternative splice site 5 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,3,
184336pDisplay-V5-Nxph1Expression of V5-tagged mouse neurexophilin-1
184337FAM19A1-V5Expression of V5-tagged mouse FAM19A1
184338FAM19A2-V5Expression of V5-tagged mouse FAM19A2

Antibodies from Article