A Robust Assay to Monitor Ataxin-3 Amyloid Fibril Assembly.
Figueiredo F, Lopes-Marques M, Almeida B, Matscheko N, Martins PM, Silva A, Macedo-Ribeiro S
Cells. 2022 Jun 19;11(12). pii: cells11121969. doi: 10.3390/cells11121969.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184246 | ATX3-D1 | Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal) |
| 184247 | ATX3-JD | Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal) |
| 184248 | ATX3-13Q | Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal) |
| 184249 | ATX3-77Q | Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal) |
| 184251 | ATX3-77Q-R388G | Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal) |
| 185908 | ATX3 13Q- I77K Q78K W87K | Expresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal). |