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Reprogrammed tracrRNAs enable repurposing of RNAs as crRNAs and sequence-specific RNA biosensors.

Liu Y, Pinto F, Wan X, Yang Z, Peng S, Li M, Cooper JM, Xie Z, French CE, Wang B
Nat Commun. 2022 Apr 11;13(1):1937. doi: 10.1038/s41467-022-29604-x. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
184138pLY162Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1)
184140pLY164Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)
184141pLY163Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2)
184142pLY168Reporter circuit for hijacking mRNA of arsenic responsive gene cluster as CRISPR RNA (site 2)
184145pLY171sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa
184146pLY185RFP generator controlled by Plux2 promoter
184148pLY227short crRNA generator with spacer LEA2 with WT repeat region
184149pLY232short tracrRNA generator with WT anti-repeat region
184150pLY237Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 1)
184152pLY239Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 3)
184153pLY251Reprogrammed short tracrRNA for hijacking mRNA of arsenic responsive gene cluster (site 2)
184155pLY273Reprogrammed short tracrRNA for hijacking mRNA of zraP
184156pLY283Reprogrammed short tracrRNA for hijacking mRNA of zntA
184158pLY300Reporter circuit for hijacking small RNA oxyS as CRISPR RNA
184159pLY305Reporter circuit for hijacking mRNA of zraP as CRISPR RNA
184160pLY315Reporter circuit for hijacking mRNA of zntA as CRISPR RNA
184161pLY320Reporter circuit for hijacking mRNA of cusCFAB as CRISPR RNA

Antibodies from Article