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The Aurora B-controlled PP1/RepoMan complex determines the spatial and temporal distribution of mitotic H2B S6 phosphorylation.

Pfisterer M, Robert R, Saul VV, Pritz A, Seibert M, Feederle R, Schmitz ML
Open Biol. 2024 May;14(5):230460. doi: 10.1098/rsob.230460. Epub 2024 May 29. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
184041pEGFP-H2B-MCSCloning of GFP-H2B fusion proteins
184042H2B-GFP-Aurora B WTExpresses H2B-GFP fused to Aurora B WT
184043H2B-GFP-Aurora B KDExpresses H2B-GFP fused to Aurora B kinase dead
184044H2B-GFP-INCENP∆cenExpresses H2B-GFP fused to INCENP∆cen
184269pEGFP-RepoMan-wtExpresses shRNA resistant GFP-RepoMan
184270pEGFP-RepoMan-RATAExpresses shRNA resistant GFP-RepoMan RATA Mutant
184272pEGFP-Repo-Man-1-890Expresses C-terminally truncated GFP-RepoMan
204994pEGFP-RepoMan-T394AExpresses shRNA resistant GFP-RepoMan T394A Mutant
204995pEGFP-RepoMan-S893DExpresses shRNA resistant GFP-RepoMan S893D Mutant

Antibodies from Article