Transcription activation is enhanced by multivalent interactions independent of phase separation.
Trojanowski J, Frank L, Rademacher A, Mucke N, Grigaitis P, Rippe K
Mol Cell. 2022 May 4. pii: S1097-2765(22)00328-8. doi: 10.1016/j.molcel.2022.04.017.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183916 | rTetR-GFP | Reverse Tet repressor labeled with GFP; binds tetO sites upon doxycycline addition |
| 183917 | rTetR-GFP-VP16 | VP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition |
| 183918 | rTetR-GFP-VPR | VPR activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition |
| 183919 | CIBN-rTetR | Optogenetic CIBN localizer domain coupled to reverse Tet repressor; binds tetO sites upon doxycycline addition; CIBN is bound by PHR upon blue light exposure |
| 183920 | GFP-LacI | Lac repressor labeled with GFP; can be used to label lacO sites |
| 183921 | tdTomato-LacI | Lac repressor labeled with tdTomato; can be used to label lacO sites |
| 183922 | SNAPtag-LacI | Lac repressor labeled with SNAPtag; can be used to label lacO sites |
| 183923 | NLS-dCas9-HA-NLS-GFP-VP16 | VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP |
| 183924 | NLS-dCas9-HA-NLS-GFP4-VPR | VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer |
| 183925 | NLS-dCas9-HA-NLS--GFP-p300 | p300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFP |
| 183926 | PHR-GFP | Optogenetic PHR domain coupled to GFP; binds to CIBN upon blue light exposure |
| 183927 | PHR-GFP-VP16 | Optogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposure |
| 183928 | PHR-GFP-VPR | Optogenetic PHR domain coupled to GFP and VPR activation domain; binds to CIBN upon blue light exposure |
| 183929 | PHR-GFP-p65 | Optogenetic PHR domain coupled to GFP and p65 activation domain; binds to CIBN upon blue light exposure |
| 183930 | PHR-GFP-Rta | Optogenetic PHR domain coupled to GFP and Rta activation domain; binds to CIBN upon blue light exposure |
| 183931 | PHR-GFP-STAT2 | Optogenetic PHR domain coupled to GFP and STAT2 activation domain; binds to CIBN upon blue light exposure |
| 183932 | PHR-GFP-FUSN | Optogenetic PHR domain coupled to GFP and FUSN domain with a high phase separation propensity; binds to CIBN upon blue light exposure |
| 183933 | PHR-GFP-FUSN-VP16 | Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure |
| 183934 | NLS-tdPCP-GFP | PCP tandem dimer binding to PP7 RNA stem loops; labeled with GFP |
| 183935 | NLS-tdPCP-GFP-VP16 | PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP |
| 183936 | NLS-tdPCP-GFP-VPR | PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP |
| 183937 | NLS-tdPCP-CIBN | PCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposure |
| 183938 | NLS-HA-tdMCP-tdTomato | tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag |
| 183939 | mCherry-BRD4 | Transcriptional co-activator BRD4 labeled by mCherry |