Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control.
Lutrat C, Burckbuchler M, Olmo RP, Beugnon R, Fontaine A, Akbari OS, Argiles-Herrero R, Baldet T, Bouyer J, Marois E
Commun Biol. 2023 Jun 16;6(1):646. doi: 10.1038/s42003-023-05030-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183903 | pX4 | donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus |
| 183904 | pX3 | donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus |
| 183911 | pENTR-attB-PUb-GFP | attB-site containing docking plasmid for genomic insertion into attP site, encoding GFP under control of the Aedes aegypti PUb promoter |
| 183912 | pKSB-AeU6-1gRNA | gRNA-expressing plasmid under Aedes aegypti AAEL017702 U6 promoter |
| 183913 | pKSB-AeU6-2gRNA | gRNA-expressing plasmid under Aedres aegypti AAEL017774 U6 promoter |
| 183914 | pKSB-AeU6-3gRNA | gRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoter |
| 183966 | pB PUb-integrase | piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker |