Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control.

Lutrat C, Burckbuchler M, Olmo RP, Beugnon R, Fontaine A, Akbari OS, Argiles-Herrero R, Baldet T, Bouyer J, Marois E
Commun Biol. 2023 Jun 16;6(1):646. doi: 10.1038/s42003-023-05030-7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
183966	pB PUb-integrase	piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker
183914	pKSB-AeU6-3gRNA	gRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoter
183913	pKSB-AeU6-2gRNA	gRNA-expressing plasmid under Aedres aegypti AAEL017774 U6 promoter
183912	pKSB-AeU6-1gRNA	gRNA-expressing plasmid under Aedes aegypti AAEL017702 U6 promoter
183911	pENTR-attB-PUb-GFP	attB-site containing docking plasmid for genomic insertion into attP site, encoding GFP under control of the Aedes aegypti PUb promoter
183904	pX3	donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus
183903	pX4	donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus