dCas9-based gene editing for cleavage-free genomic knock-in of long sequences.
Wang C, Qu Y, Cheng JKW, Hughes NW, Zhang Q, Wang M, Cong L
Nat Cell Biol. 2022 Feb;24(2):268-278. doi: 10.1038/s41556-021-00836-1. Epub 2022 Feb 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183826 | p-dCas9-SSAP-MS2-BB_BbsI | pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP |
| 183827 | pMCP-RecT | pLenti-EF1A-MCP-EXTEN-RecT-NLS |