A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis.

Davis RP, Costa M, Grandela C, Holland AM, Hatzistavrou T, Micallef SJ, Li X, Goulburn AL, Azzola L, Elefanty AG, Stanley EG
Nat Protoc. 2008;3(10):1550-8. doi: 10.1038/nprot.2008.146. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
183812	pEFBOS_CreIRESpuro	Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter