A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis.
Davis RP, Costa M, Grandela C, Holland AM, Hatzistavrou T, Micallef SJ, Li X, Goulburn AL, Azzola L, Elefanty AG, Stanley EG
Nat Protoc. 2008;3(10):1550-8. doi: 10.1038/nprot.2008.146. PubMed Article
Davis RP, Costa M, Grandela C, Holland AM, Hatzistavrou T, Micallef SJ, Li X, Goulburn AL, Azzola L, Elefanty AG, Stanley EG
Nat Protoc. 2008;3(10):1550-8. doi: 10.1038/nprot.2008.146. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183812 | pEFBOS_CreIRESpuro | Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter |