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Polycomb inhibits histone acetylation by CBP by binding directly to its catalytic domain.

Tie F, Banerjee R, Fu C, Stratton CA, Fang M, Harte PJ
Proc Natl Acad Sci U S A. 2016 Feb 9;113(6):E744-53. doi: 10.1073/pnas.1515465113. Epub 2016 Jan 22. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
183777pET11-PC-H6Express PC-H6 in E. coli. Purified PC-H6 was used for for generating antibodies in rabbits and for GST-CBP pull-down assays.
183779pET11-PC(75-228)-H6Express PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.
183780pET-PC(1-86)-H6Express PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assays
183781pET11-PC(75-390)-H6Express PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assays

Antibodies from Article