A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii.
Ito Y, Ishigami M, Terai G, Nakamura Y, Hashiba N, Nishi T, Nakazawa H, Hasunuma T, Asai K, Umetsu M, Ishii J, Kondo A
Commun Biol. 2022 Jun 8;5(1):561. doi: 10.1038/s42003-022-03475-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183732 | pPGP_scFv | Expresses anti-lysozyme scFv antibody |
| 183733 | pPAP_scFv | Expresses anti-lysozyme scFv antibody |
| 183734 | pPAP_scFv_V50A | Expresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signal |
| 183735 | pPAP_BGL1 | Expresses Aspergillus aculeatus β-glucosidase (BGL1) |
| 183736 | pREMI-ZA | Plasmid for a restriction enzyme mediated integration (REMI) in Komagataella phaffii |