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Global analysis of contact-dependent human-to-mouse intercellular mRNA and lncRNA transfer in cell culture.

Dasgupta S, Dayagi DY, Haimovich G, Wyler E, Olender T, Singer RH, Landthaler M, Gerst JE
Elife. 2023 May 30;12:e83584. doi: 10.7554/eLife.83584. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
183710pUbC-tdTomatotdTomato expression from UbiC promoter
183712Lenti-UbiC-GFP-psGFP with palmitoylation signal
183714tdTomato--β-actin CDS2tdTomato with fragment nucleotides 398-818 of beta-actin coding sequence inserted in 3'UTR
183715tdTomato-β-actinCDS3tdTomato with fragment nucleotides 801-1127 of beta-actin coding sequence inserted in 3'UTR
183716tdTomato-β-actin3'UTR1tdTomato with fragment nucleotides 1-440 of beta-actin 3'UTR inserted in 3'UTR
183717tdTomato-β-actin3'UTR2tdTomato with fragment nucleotides 441-676 of beta-actin 3'UTR inserted in 3'UTR
183718Inducible tdTomatotdTomato expression from dox-inducible promoter; plasmid encodes the TetR inducer

Antibodies from Article