Stress-induced protein disaggregation in the endoplasmic reticulum catalysed by BiP.
Melo EP, Konno T, Farace I, Awadelkareem MA, Skov LR, Teodoro F, Sancho TP, Paton AW, Paton JC, Fares M, Paulo PMR, Zhang X, Avezov E
Nat Commun. 2022 May 6;13(1):2501. doi: 10.1038/s41467-022-30238-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183686 | SP_H6_HaloK73T_KDEL_pCMV1 | Mammalian expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) |
| 183687 | pCMV_HaloK73T_cyto | Mammalian expression of cytosolic metastable Halotag/folding sensor (Cyto HaloK73T) |
| 183688 | SP_H6_Halo_WT_KDEL_pBABEpu | Retroviral expression of ER targeted H6-tagged Halotag Wild type |
| 183689 | SP_H6_Halo_K73T_KDEL_pBABEpu | Retroviral expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) |
| 183690 | SP_H6_Halo_L172Q_KDEL_pBABEpu | Retroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloL172Q) |
| 183691 | SP_H6_Halo_M21K_F86L_KDEL_pBABEpu | Retroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloM21K_F86L) |
| 183692 | pET30_Halotag_K73T_v2 | Bacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T) |
| 183693 | pET30_Halotag_K73T_Jdomain | Bacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domain |
| 183694 | pCRISPRa_CHO-Bip_gRNA1 | CRISPRa gRNA expression vector for Chinese Hamster Bip (Hspa5) |
| 183695 | pCRISPRa_all-in-one | Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective |
| 183696 | pCRISPRa_all-in-one_Bip gRNA1 | Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective |